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Pathways Recommended: Metabolic Enzyme/Protease
Results for "

enzyme activity detection

" in MedChemExpress (MCE) Product Catalog:

26

Inhibitors & Agonists

9

Fluorescent Dye

5

Biochemical Assay Reagents

7

Peptides

1

MCE Kits

Targets Recommended:
Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-W015996
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide

    pNP-GalNAc

    		 Fluorescent Dye Others
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GalNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide
  • HY-171045
    Abz-FRF(4NO2)

    		Cathepsin Biochemical Assay Reagents Others
    Abz-FRF(4NO2) is an Abz-tripeptide substrate commonly used for studying the activity of enzymes, especially cysteine proteases such as Cathepsin X .
    Abz-FRF(4NO2)
  • HY-P4426A
    Lys-Pro-AMC diTFA

    		Fluorescent Dye Others
    Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .
    Lys-Pro-AMC diTFA
  • HY-112688
    H-Arg-4MβNA

    		Ser/Thr Protease Others
    H-Arg-4MβNA is a substrate for cathepsin H, used for the detection of enzyme activity in gel electrophoresis.
    H-Arg-4MβNA
  • HY-W011985
    H-Arg-NH2 hydrochloride

    		Biochemical Assay Reagents Others
    H-Arg-NH2 hydrochloride is a nucleophilic reagent. H-Arg-NH2 hydrochloride can be used in experiments related to the detection of enzyme activity centers .
    H-Arg-NH2 hydrochloride
  • HY-P4426
    Lys-Pro-AMC

    		Fluorescent Dye Others
    Lys-Pro-AMC is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .
    Lys-Pro-AMC
  • HY-135256
    Naphthol yellow S, 98%

    Acid yellow 1, 98%

    		 Biochemical Assay Reagents Others
    Naphthol yellow S, 98% (Acid yellow 1, 98%) is a basic protein-binding dye used for enzyme activity detection.
    Naphthol yellow S, 98%
  • HY-137858
    H-Gly-Arg-pNA hydrochloride

    Gly-Arg-4-NA hydrochloride; GR-pNA hydrochloride; GR p-itoailide hydrochloride

    		 Fluorescent Dye Others
    H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity .
    H-Gly-Arg-pNA hydrochloride
  • HY-W927376
    Boc-Val-Arg-AMC

    		Fluorescent Dye Others
    Boc-Val-Arg-AMC is a synthetic peptide compound. As a specific fluorescent substrate, Boc-Val-Arg-AMC can be specifically cleaved by specific enzymes, especially thrombin, to release a fluorescent molecule AMC (7-amino-4-methylcoumarin), which can be used to monitor thrombin activity. Boc-Val-Arg-AMC can be used to detect thrombin activity in plasma samples .
    Boc-Val-Arg-AMC
  • HY-D0146
    Resorufin benzyl ether

    BzRes; 7-Benzyloxyresorufin; 7-Benzyloxyphenoxazone

    		 Fluorescent Dye Others
    Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO - detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm .
    Resorufin benzyl ether
  • HY-131618
    Hippuryl-Arg

    Bz-Gly-Arg

    		 Amino Acid Derivatives Others
    Hippuryl-Arg (Bz-Gly-Arg) is a substrate used for fluorescence detection and is commonly used in biochemical studies, especially in studying enzyme activity. The phenylalanine portion of Hippuryl-Arg is connected by an aromatic side chain, which allows it to be detected under ultraviolet light. Hippuryl-Arg is used to detect enzymes that recognize and cut peptide bonds containing arginine .
    Hippuryl-Arg
  • HY-143285
    AMC-GlcNAc

    		Fluorescent Dye Infection
    AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .
    AMC-GlcNAc
  • HY-W013168
    4-Nitrophenyl palmitate

    4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp

    		 Bacterial Infection
    4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate) is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-Nitrophenyl palmitate, 4-nitrophenol is released, which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipase.
    4-Nitrophenyl palmitate
  • HY-137855
    4-Methylumbelliferyl sulfate potassium

    		Biochemical Assay Reagents Others
    4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
    4-Methylumbelliferyl sulfate potassium
  • HY-120833
    Ac-Ile-Glu-Thr-Asp-pNA

    		Caspase Others
    Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .
    Ac-Ile-Glu-Thr-Asp-pNA
  • HY-P4551
    Hippuryl-His-Leu-OH

    N-Benzoyl-Gly-His-Leu

    		 Angiotensin-converting Enzyme (ACE) Amino Acid Derivatives Cardiovascular Disease
    Hippuryl-His-Leu-OH (N-Benzoyl-Gly-His-Leu) is a substrate for detecting the activity of angiotensin I converting enzyme. The His-Leu released by Hippuryl-His-Leu-OH can react with o-phtbaldialdehyde or Fluorescamine (HY-D0715) for fluorescence detection .
    Hippuryl-His-Leu-OH
  • HY-137875
    Hippuryl-His-Leu-OH hydrate

    N-Benzoyl-Gly-His-Leu hydrate

    		 Angiotensin-converting Enzyme (ACE) Amino Acid Derivatives Cardiovascular Disease
    Hippuryl-His-Leu-OH (N-Benzoyl-Gly-His-Leu) hydrate is a substrate for detecting the activity of angiotensin I converting enzyme. The His-Leu released by Hippuryl-His-Leu-OH hydrate can react with o-phtbaldialdehyde or Fluorescamine (HY-D0715) for fluorescence detection .
    Hippuryl-His-Leu-OH hydrate
  • HY-W854392
    3-Carboxyumbelliferyl-β-D-glucuronide dipotassium

    		Biochemical Assay Reagents
    3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) is a chromogenic substrate with biological activity for enzyme activity detection. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) can be used to evaluate biological reactions associated with glycosidases. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) shows a wide range of application potentials in compound discovery and biomedical research.
    3-Carboxyumbelliferyl-β-D-glucuronide dipotassium
  • HY-114879
    DDAO
    1 Publications Verification

    		 Fluorescent Dye Carboxylesterase (CES) Cancer
    DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases .
    DDAO
  • HY-118210
    PHOP

    		FAAH Metabolic Disease
    PHOP is a fatty acid amide hydrolase (FAAH) inhibitor used to assess inhibitory activity in a fluorometric assay. PHOP can determine FAAH activity by measuring the amount of 4-pyridin-1-ylbutyric acid released by the enzyme in rat brain microsomes. PHOP demonstrates potential as a FAAH inhibitor and can directly measure FAAH activity by reversed-phase HPLC and fluorescence detection, providing a basis for the development of new inhibitors.
    PHOP
  • HY-134429
    p-Nitrophenyl α-L-arabinopyranoside

    		Biochemical Assay Reagents Others
    p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection .
    p-Nitrophenyl α-L-arabinopyranoside
  • HY-W928283
    L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride

    		Biochemical Assay Reagents
    L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride is a fluorescent substrate with biological activity for enzyme activity detection. L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride is often used in biochemical research to detect reactions associated with specific enzymes. L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride helps scientists monitor the progress of reactions through its fluorescent properties. L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride has important application value in compound development and basic biological research.
    L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride
  • HY-D0027
    7-Amino-4-methylcoumarin

    Coumarin 120; AMC

    		 Fluorescent Dye Antibiotic Fungal Infection
    7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively.
    7-Amino-4-methylcoumarin
  • HY-D0027R
    7-Amino-4-methylcoumarin (Standard)

    Coumarin 120 (Standard); AMC (Standard)

    		 Fluorescent Dye Antibiotic Fungal Reference Standards Infection
    7-Amino-4-methylcoumarin (Standard) is the analytical standard of 7-Amino-4-methylcoumarin. This product is intended for research and analytical applications. 7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively. [4]
    7-Amino-4-methylcoumarin (Standard)
  • HY-W010991
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly

    FAPGG

    		 Angiotensin-converting Enzyme (ACE) Others
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly (FAPGG) is a specific substrate of angiotensin converting enzyme (ACE) with a Ki of 2.546×10 -4 M. It is used as a chromogenic probe for quantitative detection of ACE activity. N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly can be hydrolyzed by ACE to generate N-[3-(2-furyl)acryloyl]-Phe (FAP) and Gly-Gly, and the ACE inhibitory effect is monitored by photometry. FAPGG competitively binds to the active center of ACE and is a key tool for screening ACE inhibitors such as Captopril (HY-B0368) and Dioscorin. Its reversible mechanism of action supports hypertension research and drug development targeting the renin-angiotensin system .
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly
  • HY-137276
    5-Bromo-3-indolyl β-D-galactopyranoside

    Bluo-Gal

    		 Fluorescent Dye Glycosidase Others
    5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues .
    5-Bromo-3-indolyl β-D-galactopyranoside

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