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BPFluor 405 acid is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The carboxylic acid of BPFluor 405 is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU. BPFluor 405 acid is also often used for control experiments, and for calibration.
BPFluor 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BPFluor 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. BPFluor 405 conjugates can also be used for the detection of abundance targets.
BPFluor 405 Cadaverine is a carbonyl-reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. Another common application for BPFluor 405 Cadaverine is cell fixing by treatment with formaldehyde or glutaraldehyde.
BPFluor 405 is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode.
BPFluor 405 alkyne triTEA is a blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (BPFluor 405 DBCO) is also available for application where the presence of copper is not acceptable.
BPFluor 405 DBCO is a blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 DBCO reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.
BPFluor 405 Picolyl Azide is an advanced fluorescent probe that incorporatess a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of the CuAAC reaction depends on the concentrations of all reagents, including copper, therefore raising the effective copper concentration at the reaction site dramatically increases the rate of CuAAC reaction without increasing the concentrations of azide reagent or copper.
In addition, the use of picolyl azide instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This is of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
BP Fluor 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
BPFluor 405 acid is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The carboxylic acid of BPFluor 405 is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU. BPFluor 405 acid is also often used for control experiments, and for calibration.
BPFluor 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BPFluor 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. BPFluor 405 conjugates can also be used for the detection of abundance targets.
BPFluor 405 Cadaverine is a carbonyl-reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. Another common application for BPFluor 405 Cadaverine is cell fixing by treatment with formaldehyde or glutaraldehyde.
BPFluor 405 is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode.
BPFluor 405 alkyne triTEA is a blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (BPFluor 405 DBCO) is also available for application where the presence of copper is not acceptable.
BPFluor 405 DBCO is a blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 DBCO reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.
BPFluor 405 Picolyl Azide is an advanced fluorescent probe that incorporatess a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of the CuAAC reaction depends on the concentrations of all reagents, including copper, therefore raising the effective copper concentration at the reaction site dramatically increases the rate of CuAAC reaction without increasing the concentrations of azide reagent or copper.
In addition, the use of picolyl azide instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This is of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
BP Fluor 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
BPFluor 405 alkyne triTEA is a blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BPFluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (BPFluor 405 DBCO) is also available for application where the presence of copper is not acceptable.
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