RNF5 inhibitor inh-02

Name: RNF5 inhibitor inh-02
Brand: MedChemExpress
SKU: HY-123967
Rating: 4.5 (1 reviews)

Cat. No.: HY-123967 Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

RNF5 inhibitor inh-02 is a potent inhibitor of E3 ubiquitin ligase RNF5/RMA1. RNF5 inhibitor inh-02 rescues F508del-CFTR function in F508del-CFTR-expressing immortalized cells (CFBE41o⁻, EC₅₀ = 2.6 μM; FRT, EC ₅₀ = 2.2 μM). RNF5 inhibitor inh-02 increases LC3IIB expression and autophagic vacuole number via reducing ATG4B ubiquitylation and promotes cell motility. RNF5 inhibitor inh-02 can be used for the study of cystic fibrosis.

For research use only. We do not sell to patients.

RNF5 inhibitor inh-02 Chemical Structure

CAS No. : 324579-65-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

RNF5 inhibitor inh-02 (2-10 μM, 24 h) significantly reduces F508del-CFTR ubiquitylation and rescues F508del-CFTR activity (EC₅₀ = 2.6 μM) in CFBE41o^- cells stably expressing F508del-CFTR and HS-YFP^[1].
RNF5 inhibitor inh-02 (1-10 μM, 24 h) dose-dependently rescues F508del-CFTR activity in F508del-CFTR/HS-YFP-expressing FRT cells with an EC₅₀ of 2.2 μM, and increases CFTR-dependent transepithelial conductance^[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) significantly increases CFTR-mediated transepithelial conductance, equivalent short-circuit current and chloride secretion in primary bronchial epithelial cells^[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) reduces ATG4B ubiquitylation, increases LC3IIB expression and autophagic vacuole number in CFBE41o^- cells^[1].
RNF5 inhibitor inh-02 (5 μM, 24 h) promotes cell migration and accelerates scratch gap filling in CFBE41o^- cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

384.50

Formula

C₂₃H₂₀N₄S

CAS No.

324579-65-9

Appearance

Solid

Color

White to yellow

SMILES

CC1=NS/C(N1CC2=CC=CC=C2)=N\C(C3=CC=CC=C3)=N\C4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (216.72 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6008 mL	13.0039 mL	26.0078 mL
5 mM	0.5202 mL	2.6008 mL	5.2016 mL
10 mM	0.2601 mL	1.3004 mL	2.6008 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (278 KB) SDS (614 KB)

COA (257 KB) HNMR (140 KB) RP-HPLC (140 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Elvira Sondo, et al. Pharmacological Inhibition of the Ubiquitin Ligase RNF5 Rescues F508del-CFTR in Cystic Fibrosis Airway Epithelia. Cell Chem Biol. 2018 Jul 19;25(7):891-905.e8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6008 mL	13.0039 mL	26.0078 mL	65.0195 mL
	5 mM	0.5202 mL	2.6008 mL	5.2016 mL	13.0039 mL
	10 mM	0.2601 mL	1.3004 mL	2.6008 mL	6.5020 mL
	15 mM	0.1734 mL	0.8669 mL	1.7339 mL	4.3346 mL
	20 mM	0.1300 mL	0.6502 mL	1.3004 mL	3.2510 mL
	25 mM	0.1040 mL	0.5202 mL	1.0403 mL	2.6008 mL
	30 mM	0.0867 mL	0.4335 mL	0.8669 mL	2.1673 mL
	40 mM	0.0650 mL	0.3251 mL	0.6502 mL	1.6255 mL
	50 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3004 mL
	60 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0837 mL
	80 mM	0.0325 mL	0.1625 mL	0.3251 mL	0.8127 mL
	100 mM	0.0260 mL	0.1300 mL	0.2601 mL	0.6502 mL