2. Recombinant Proteins
  3. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  4. TGF-beta Superfamily Receptor Serine/Threonine Kinases Serine/Threonine Kinase Proteins
  5. Activin/Inhibins Receptor
  6. ALK-4/Activin RIB
  7. RIB/ALK-4 Protein, Mouse (344a.a, HEK293, Fc )

RIB/ALK-4 Protein, Mouse (344a.a, HEK293, Fc )

Cat. No.: HY-P703927
Handling Instructions Technical Support

RIB/ALK-4 Protein, Mouse (344a.a, HEK293, Fc ) is the recombinant mouse-derived RIB/ALK-4 protein, expressed by HEK293, with C-hFc tag.

For research use only. We do not sell to patients.

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Description

RIB/ALK-4 Protein, Mouse (344a.a, HEK293, Fc ) is the recombinant mouse-derived RIB/ALK-4 protein, expressed by HEK293, with C-hFc tag.
Background

RIB/ALK-4 is a transmembrane serine/threonine kinase activin type-1 receptor that forms an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). It transduces activin signals from the cell surface to the cytoplasm, regulating various physiological and pathological processes, including neuronal differentiation and survival, hair follicle development and cycling, pituitary FSH production, wound healing, extracellular matrix production, immunosuppression, and carcinogenesis. Activin is also implicated in paracrine or autocrine roles during ovarian follicular development. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) serve as primary activin receptors, while type-1 receptors like ACVR1B act as downstream transducers. Activin binds to type-2 receptors at the plasma membrane, activating their serine-threonine kinase activity, which then phosphorylates and activates type-1 receptors such as ACVR1B. Activated type-1 receptors phosphorylate SMAD2 and SMAD3 at C-terminal serine residues, leading to their release into the cytoplasm, where they form a complex with SMAD4. This SMAD complex translocates to the nucleus to mediate activin-induced transcription. Inhibitory SMAD7, recruited to ACVR1B via FKBP1A, blocks activin signaling by preventing SMAD2/SMAD3 association with the receptor complex. Additionally, inhibin-B binding via the IGSF1 inhibin coreceptor antagonizes activin signaling. ACVR1B also phosphorylates TDP2.
Species

Mouse
Source

HEK293
Tag

C-hFc
Accession

NP_031421.1 (M1-E126)
Gene ID

11479
Molecular Construction
N-term
RIB/ALK-4 (M1-E126)
Accession # NP_031421.1
hFc
C-term
Protein Length

Partial
Synonyms
6820432J04 Protein; ActR-IB Protein; ActRIB; Acvr1b Protein; Acvrlk4 Protein; ALK-4 Protein; Alk4 Protein; SKR2 Protein
AA Sequence

MAESAGASSFFPLVVLLLAGSGGSGPRGIQALLCACTSCLQTNYTCETDGACMVSIFNLDGVEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYIDFCNKIDLRVPSGHLKEPAHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEDYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNVPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI

Predicted Molecular Mass
38.4 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

RIB/ALK-4 Protein, Mouse (344a.a, HEK293, Fc ) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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