MMP-10 Protein, Human (P. pastoris, His)

MMP-10 protein is a member of the matrix metalloproteinase family and plays a crucial role in extracellular matrix remodeling by promoting the degradation of various substrates. As a protease, MMP-10 is involved in tissue homeostasis, wound healing, and inflammatory responses. MMP-10 Protein, Human (P. pastoris, His) is the recombinant human-derived MMP-10 protein, expressed by P. pastoris , with N-6*His labeled tag.

MMP-10, also known as Stromelysin-2 or transin-2, is classified into the simple hemopexin domain structural class. MMP-10 has a relatively broad substrate specificity, including proMMPs, ECM proteins, proteoglycans, glycoproteins, and collagens. Unlike several other MMPs that are localised predominantly in tumor stroma, tumor cells themselves appear to express MMP-10. MMP-10 is a potential tumor marker^[1].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

P09238 (F99-C476)

4319  [NCBI]

Full Length of Mature Protein

FSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVWEEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVPSGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDTVFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQGFPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC

45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jason H Gill, et al. MMP-10 is overexpressed, proteolytically active, and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia. 2004 Nov-Dec;6(6):777-85.  [Content Brief]