IL1R1 Protein, Cynomolgus (HEK293, His)

IL1R1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL1R1 protein, expressed by HEK293, with C-His tag.

IL1R1, a receptor for interleukin-1A (IL1A), interleukin-1B (IL1B), and interleukin-1 receptor antagonist (IL1RN), forms a high-affinity complex with the coreceptor IL1RAP upon ligand binding. This complex mediates interleukin-1-dependent activation of signaling pathways such as NF-kappa-B and MAPK. The signaling cascade involves the recruitment of adapter molecules, including TOLLIP, MYD88, and IRAK1 or IRAK2, through the TIR domains of the receptor/coreceptor subunits. IL1R1 binds ligands with comparable affinity, and the interaction with antagonist IL1RN prevents the association with IL1RAP, thus inhibiting the formation of a signaling complex. Furthermore, IL1R1 is implicated in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.

Cynomolgus

HEK293

C-His

EHH61701.1 (M1-T332)

/

Partial

Approximately 47-57 kDa,based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.