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  4. Coagulation Factor II Protein Protein, Rat (HEK293, His)

Coagulation Factor II Protein Protein, Rat (HEK293, His)

Cat. No.: HY-P703740
Handling Instructions Technical Support

Coagulation Factor II Protein Protein, Rat (HEK293, His) is the recombinant rat-derived Coagulation Factor II protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Description

Coagulation Factor II Protein Protein, Rat (HEK293, His) is the recombinant rat-derived Coagulation Factor II protein, expressed by HEK293, with C-His tag.
Background

Coagulation Factor II, also known as Thrombin, cleaves peptide bonds after Arg and Lys, converting fibrinogen to fibrin and activating factors V, VII, VIII, XIII, and protein C in complex with thrombomodulin. It plays a central role in blood homeostasis, inflammation, and wound healing. Additionally, it activates coagulation factor XI (F11), with activation enhanced by contact with negatively charged surfaces. In endothelial cells, it triggers the production of pro-inflammatory cytokines such as MCP-1/CCL2 and IL8/CXCL8.
Species

Rat
Source

HEK293
Tag

C-8*His
Accession

NP_075213 (Q25-R617)
Protein Length

Full Length of Mature Protein
Predicted Molecular Mass
69.1 kDa
Molecular Weight

Approximately 70-80 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Coagulation Factor II Protein Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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