2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. MME Protein, Cynomolgus (HEK293, His)

MME Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived MME protein, expressed by HEK293, with N-His tag.

For research use only. We do not sell to patients.

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Description

MME Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived MME protein, expressed by HEK293, with N-His tag.
Background

The MME Protein demonstrates a thermolysin-like specificity, predominantly acting on polypeptides of up to 30 amino acids. Biologically crucial, it plays a key role in the degradation of opioid peptides, including Met- and Leu-enkephalins, achieved through the cleavage of a Gly-Phe bond. Additionally, MME catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides and is capable of cleaving angiotensin-1, angiotensin-2, and angiotensin 1-9. The protein is involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Furthermore, MME displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Species

Cynomolgus
Source

HEK293
Tag

N-8*His
Accession

G7NZK8 (D53-W750)
Gene ID

101925772
Protein Length

Partial
Synonyms
Neprilysin; Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; Skin fibroblast elastase; MME;
Predicted Molecular Mass
80.7 kDa
Molecular Weight

Approximately 81-110 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by Bis-Tris PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MME Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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