Proteinase K (NGS grade)  (Synonyms: Protease K (NGS grade))

Proteinase K (Protease K) (NGS grade) (EC 3.4.21.64) is a serine protease with high enzyme activity and broad substrate specificity. Proteinase K can preferentially decompose ester bonds and peptide bonds adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. This product is NGS grade, no Nickase residue, and nucleic acid residue ≤5pg/mg^[1].

Product Information
The product can be used for 1. Genetic diagnostic kits; 2.RNA and DNA extraction kits; 3. Extract non-protein components from tissues and degrade protein-like impurities, such as the preparation of DNA vaccines and heparin; 4. Prepare chromosomal DNA for pulse electrophoresis; 5. Western blotting 6. Research and development as well as large-scale production of enzymatic glycated albumin reagents for in vitro diagnostics.

Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 7.81
Optimal pH: 7.0-12.0
Optimal temperature: 65°C
pH stability: pH 4.5-12.5 (25°C, 16 hours)
Thermal stability: Stable below 50°C (pH 8.0, 30 min)
Storage stability: Viability exceeds 90% within one year at 25°C
Activators: SDS (HY-K1030), Urea (HY-Y0271)
Inhibitors: Diisopropyl fluorophosphate (DFP), PMSF (HY-B0496)

Instructions
It can be dissolved in water at a concentration of 1mg/mL, or it can be dissolved according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

39450-01-6

Solid

White to off-white

[Proteinase K (NGS grade)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. W Ebeling, et al. Proteinase K From Tritirachium Album Limber. Eur J Biochem . 1974 Aug 15;47(1):91-7.  [Content Brief]