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Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia .

For research use only. We do not sell to patients.

Pimonidazole Chemical Structure

Pimonidazole Chemical Structure

CAS No. : 70132-50-2

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Based on 4 publication(s) in Google Scholar

Other Forms of Pimonidazole:

Pimonidazole Related Antibodies

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Description

Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor[1]. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia [2].

In Vitro

Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
Detection: Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pimonidazole Related Antibodies
Clinical Trial
Molecular Weight

254.29

Formula

C11H18N4O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC(CN1C=CN=C1[N+]([O-])=O)CN2CCCCC2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (196.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (3.93 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9325 mL 19.6626 mL 39.3252 mL
5 mM 0.7865 mL 3.9325 mL 7.8650 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.32%

SDS (392 KB)

COA (248 KB) HNMR (260 KB) RP-HPLC (256 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.9325 mL 19.6626 mL 39.3252 mL 98.3130 mL
DMSO 5 mM 0.7865 mL 3.9325 mL 7.8650 mL 19.6626 mL
10 mM 0.3933 mL 1.9663 mL 3.9325 mL 9.8313 mL
15 mM 0.2622 mL 1.3108 mL 2.6217 mL 6.5542 mL
20 mM 0.1966 mL 0.9831 mL 1.9663 mL 4.9156 mL
25 mM 0.1573 mL 0.7865 mL 1.5730 mL 3.9325 mL
30 mM 0.1311 mL 0.6554 mL 1.3108 mL 3.2771 mL
40 mM 0.0983 mL 0.4916 mL 0.9831 mL 2.4578 mL
50 mM 0.0787 mL 0.3933 mL 0.7865 mL 1.9663 mL
60 mM 0.0655 mL 0.3277 mL 0.6554 mL 1.6385 mL
80 mM 0.0492 mL 0.2458 mL 0.4916 mL 1.2289 mL
100 mM 0.0393 mL 0.1966 mL 0.3933 mL 0.9831 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pimonidazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pimonidazole70132-50-2OthersInhibitorinhibitorinhibit

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