Pep-1 (uncapped) 

Pep-1 (uncapped) is a specific ligand of IL-13Ra2. Pep-1 (uncapped) exerts cell penetrating activity through receptor-mediated endocytosis, can cross the blood-tumor barrier (BTB) and target glioma cells. Pep-1 (uncapped) can enhance the uptake of nanoparticles by tumor cells and enhance the cell penetration ability of nanoparticles, and can be used to develop targeted drug delivery systems for glioma^[1].

Pep-1 (uncapped) enters the hydrophobic region of biological membranes and interacts more strongly with the phosphate groups of normal cell membrane vesicles and the carbonyl groups of cancer cell membrane vesicles^[1].
Pep-1 (uncapped) (200 μg/mL; 4 h) enhances nanoparticle uptake through IL-13Ra2-mediated endocytosis in C6 glioma cells, and the cell fluorescence intensity is 1.55 times higher than that of unmodified nanoparticles^[2].
Pep-1 (uncapped) (400 μg/mL; 4 h) enables nanoparticle penetration depth of 120 μm in C6 glioma 3D spheroids^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pep-1 (uncapped) enhances the glioma targeting of nanoparticles through IL-13Ra2-mediated endocytosis and can carry payloads for targeted delivery to biological tissues. Pep-1 (uncapped) loaded with coumarin-6 (0.15 mg/kg; intravenous injection; 1 time; 1 h) treats the intracranial C6 glioma model in mice. The distribution amount of coumarin-6 in glioma tissue is 2.21 times that of the non-targeted nanoparticle delivery group, and the distribution amount in normal brain tissue is 1.55 times^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2848.22

C₁₃₆H₁₉₅N₃₅O₃₃

395069-86-0

Solid

White to off-white

Lys-Glu-Thr-Trp-Trp-Glu-Thr-Trp-Trp-Thr-Glu-Trp-Ser-Gln-Pro-Lys-Lys-Lys-Arg-Lys-Val

KETWWETWWTEWSQPKKKRKV

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Almarwani B, et al. Vesicles mimicking normal and cancer cell membranes exhibit differential responses to the cell-penetrating peptide Pep-1. Biochim Biophys Acta Biomembr. 2018 Jun;1860(6):1394-1402.  [Content Brief]

  [2]. Wang B, et al. Nanoparticles functionalized with Pep-1 as potential glioma targeting delivery system via interleukin 13 receptor α2-mediated endocytosis. Biomaterials. 2014 Jul;35(22):5897-907.  [Content Brief]