para-Nitroblebbistatin 

para-Nitroblebbistatin is a derivative of Blebbistatin (HY-13813) and an inhibitor of myosin II. para-Nitroblebbistatin is photostable, non-cytotoxic, and non-phototoxic. para-Nitroblebbistatin can serve as an ideal substitute for Blebbistatin (HY-13813) to study the role of myosin II in physiology, development, and cell biology^[1][2].

para-Nitroblebbistatin (0.31-10 μM; 24 h) can inhibit cytokinesis in COS-7 cells, resulting in a multinucleated phenotype in the cells, with an EC₅₀ of 2.9 μM^[1].
para-Nitroblebbistatin (10 μM; irradiated with 480 nM for 15 min; 18 h) is non-cytotoxic in HeLa cells, and there are no differences in cell mortality and morphology compared with the control group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

para-Nitroblebbistatin (5-20 μM; single dose; 24 h) can inhibit the cell migration of the posterior lateral line primordium in transgenic cldnb:gfp zebrafish embryos, causing the body morphology of zebrafish embryos to be curved. This effect is similar to that of Blebbistatin (HY-13813), but the animal mortality rate in the para-Nitroblebbistatin group is significantly reduced^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

337.33

C₁₈H₁₅N₃O₄

1621326-32-6

Solid

Yellow to orange

O=C1[C@]2(C(N(CC2)C3=CC=C(C=C3)[N+]([O-])=O)=NC4=C1C=C(C=C4)C)O

Room temperature in continental US; may vary elsewhere.

• [1]. Radnai L, et al. A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitors. J Biol Methods. 2020 Sep 17;7(3):e136.  [Content Brief]

  [2]. Képiró M, et al. para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor. Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5.  [Content Brief]