PAN endonuclease-IN-3 

PAN endonuclease-IN-3 is a potent PAN endonuclease inhibitor that against influenza virus polymerase complexes with an IC₅₀ of 17.4 nM. PAN endonuclease-IN-3 demonstrates robust antiviral activities against multiple current and different influenza virus strains while showing minimal cytotoxicity in MDCK cells. PAN endonuclease-IN-3 significantly suppresses viral replication in an A/WSN/33 infected mouse model^[1].

PAN endonuclease-IN-3 (Compound 39-(S)) (1-10 μM) exhibits influenza A Virus (IAV) polymerase inhibitory potency in HEK-293T cells with an IC₅₀ of 17.4 nM, which is comparable activity to Baloxavir (HY-109025A) (IC₅₀ = 17.8 nM)^[1].

PAN endonuclease-IN-3(1-103 nM) demonstrates potent inhibitory activities against PAN endonuclease with an IC₅₀ value of 41.8 nM^[1].

PAN endonuclease-IN-3 (46 μL/well, 72 h) demonstrates exceptional antiviral activities with an EC₅₀ value of 0.21 nM in Madin-Darby canine kidney (MDCK) cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PAN endonuclease-IN-3 (20 mg/kg, intranasal administration, single dose) suppresses virus replication in the lungs of A/WSN/33 virus-infected mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

511.54

C₂₆H₂₃F₂N₃O₄S

CC(C)N(C1(COC1)N([C@@H]2C3=C(SCC4=C(C(F)=CC=C42)F)C=CC=C3)N5C=C6)C(C5=C(O)C6=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Liu S, et al. Design and Synthesis of PAN Endonuclease Inhibitors through Spirocyclization Strategy against Influenza A Virus. J Med Chem. 2025 Jun 18.  [Content Brief]