PA Janelia Fluor® 549, SE  (Synonyms: PA-JF549-NHS)

PA Janelia Fluor 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm^[1][2].

PA Janelia Fluor is the integration of a four-member azetidine ring, which reduces the photobleaching and photodamage, improves the brightness and photostability of the fluorescent dye. PA Janelia Fluor exhibits cell membrane permeability that can be used for live cell imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

575.57

C₃₂H₂₅N₅O₆

1811539-42-0

Solid

Light yellow to orange

549

505

O=C(C1=CC=C(C(C23C4=CC=C(C=C4OC5=CC(N6CCC6)=CC=C35)N7CCC7)=C1)C(C2=[N+]=[N-])=O)ON8C(CCC8=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188.  [Content Brief]

  [2]. Jonathan B Grimm, et al. Bright photoactivatable fluorophores for single-molecule imaging. Nat Methods. 2016 Dec;13(12):985-988.  [Content Brief]