2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. p-Nitrophenyl phosphorylcholine

p-Nitrophenyl phosphorylcholine  (Synonyms: 4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O-(4-Nitrophenylphosphoryl)choline)

Cat. No.: HY-100045 Purity: 99.61%
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Handling Instructions Technical Support

p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

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p-Nitrophenyl phosphorylcholine

p-Nitrophenyl phosphorylcholine Chemical Structure

CAS No. : 21064-69-7

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p-Nitrophenyl phosphorylcholine Related Antibodies

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Description

p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

Molecular Weight

304.24

Formula

C11H17N2O6P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(OCC[N+](C)(C)C)([O-])OC1=CC=C([N+]([O-])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (82.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2869 mL 16.4344 mL 32.8688 mL
5 mM 0.6574 mL 3.2869 mL 6.5738 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2869 mL 16.4344 mL 32.8688 mL 82.1720 mL
5 mM 0.6574 mL 3.2869 mL 6.5738 mL 16.4344 mL
10 mM 0.3287 mL 1.6434 mL 3.2869 mL 8.2172 mL
15 mM 0.2191 mL 1.0956 mL 2.1913 mL 5.4781 mL
20 mM 0.1643 mL 0.8217 mL 1.6434 mL 4.1086 mL
25 mM 0.1315 mL 0.6574 mL 1.3148 mL 3.2869 mL
30 mM 0.1096 mL 0.5478 mL 1.0956 mL 2.7391 mL
40 mM 0.0822 mL 0.4109 mL 0.8217 mL 2.0543 mL
50 mM 0.0657 mL 0.3287 mL 0.6574 mL 1.6434 mL
60 mM 0.0548 mL 0.2739 mL 0.5478 mL 1.3695 mL
80 mM 0.0411 mL 0.2054 mL 0.4109 mL 1.0271 mL
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p-Nitrophenyl phosphorylcholine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

p-Nitrophenyl phosphorylcholine21064-69-74-Nitrophenylphosphorylcholine 4-Nitrophenylphosphorylcholine O-(4-Nitrophenylphosphoryl)cholineEndogenous MetaboliteInhibitorinhibitorinhibit

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