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  4. Oregon green 488 azide

Oregon green 488 azide  (Synonyms: Difluorocarboxyfluorescein azide, 6-isomer)

Cat. No.: HY-D2769 Purity: 98.00%
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Oregon green 488 azide (Difluorocarboxyfluorescein azide, 6-isomer) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

For research use only. We do not sell to patients.

Oregon green 488 azide

Oregon green 488 azide Chemical Structure

CAS No. : 2648246-11-9

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Description

Oregon green 488 azide (Difluorocarboxyfluorescein azide, 6-isomer) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Molecular Weight

637.67

Formula

C33H37F2N5O6
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

[N-]=[N+]=NCCCCCCNC(C1=CC=C2C(OC3(C2=C1)C4=CC(F)=C(C=C4OC5=CC(O)=C(C=C53)F)O)=O)=O.CCN(CC)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (78.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5682 mL 7.8410 mL 15.6821 mL
5 mM 0.3136 mL 1.5682 mL 3.1364 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5682 mL 7.8410 mL 15.6821 mL 39.2052 mL
5 mM 0.3136 mL 1.5682 mL 3.1364 mL 7.8410 mL
10 mM 0.1568 mL 0.7841 mL 1.5682 mL 3.9205 mL
15 mM 0.1045 mL 0.5227 mL 1.0455 mL 2.6137 mL
20 mM 0.0784 mL 0.3921 mL 0.7841 mL 1.9603 mL
25 mM 0.0627 mL 0.3136 mL 0.6273 mL 1.5682 mL
30 mM 0.0523 mL 0.2614 mL 0.5227 mL 1.3068 mL
40 mM 0.0392 mL 0.1960 mL 0.3921 mL 0.9801 mL
50 mM 0.0314 mL 0.1568 mL 0.3136 mL 0.7841 mL
60 mM 0.0261 mL 0.1307 mL 0.2614 mL 0.6534 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oregon green 488 azide2648246-11-9Difluorocarboxyfluorescein azide, 6-isomerBiochemical Assay ReagentsInhibitorinhibitorinhibit

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