2. Others
  3. Biochemical Assay Reagents
  4. Orcein

Orcein is an irreversible stain that specifically targets elastic fibers and can interact hydrophobically with the protein components in elastic fibers. Orcein makes elastic fibers in tissues appear purple or purple-red. Orcein can be used for morphological studies of Drosophila polytene chromosomes and for qualitative and quantitative analysis of elastic fibers, collagen fibers and other components in atherosclerotic plaques.

For research use only. We do not sell to patients.

Orcein

Orcein Chemical Structure

CAS No. : 1400-62-0

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Orcein Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Orcein is an irreversible stain that specifically targets elastic fibers and can interact hydrophobically with the protein components in elastic fibers. Orcein makes elastic fibers in tissues appear purple or purple-red. Orcein can be used for morphological studies of Drosophila polytene chromosomes and for qualitative and quantitative analysis of elastic fibers, collagen fibers and other components in atherosclerotic plaques[1][2].

In Vitro

Orcein dyes bind to negatively charged groups of chromatin or through hydrophobic interactions under acidic conditions, staining chromosomes. Orcein is mainly used to stain polytene chromosomes in the salivary glands of third-instar larvae of Drosophila. It can also be used to observe chromosomes in tissues such as the midgut, hindgut, and fat body[1].
Orcein can be used to stain tissue samples. In histology and cell biology, it is mainly used to stain elastic fibers, collagen, nucleic acids, and cellular components[2].

Aceto-orcein staining[1]:
When staining with Orcein, adding acetic acid to fix the chromosomes can stretch the chromosomes in the interband region when pressing the slides, improving the band resolution; the addition of lactic acid can soften the glands and promote chromosome spreading.
1. Materials
Culture medium: Texas banana agar, yeast-glucose-agar, Carolina instant Drosophila feed.
Buffer/fixative: Drosophila Ringers solution, PBS, 0.8% NaCl, 45% acetic acid.
Staining solution: lactic acid-acetic acid-orcein staining solution (prepared according to Lim's method: 1 g natural orcein dissolved in 50 mL lactic acid, filtered; 1 g natural orcein dissolved in 50 mL glacial acetic acid, heated and filtered; the three were mixed at a ratio of 1:1:1).
Consumables: No. 5 tweezers, siliconized coverslips, coated slides, Probe-On-Plus slides, Permount mounting medium.
2. Operation steps
Larva culture and selection:
Use the above culture medium to culture third-instar larvae. Cultivate at 18°C to obtain larger chromosomes. Select larvae that have not pupated and are full-bodied, and rinse the culture medium on the body surface with PBS or Ringers solution.
Dissection and gland separation:
Place the larvae on a slide containing 45% acetic acid. Under a dissecting microscope, use a pair of forceps to clamp the head and tail respectively, and pull the head off quickly to expose the salivary gland (a transparent long cyst-like structure with attached adipose tissue).
Strip the gland, remove the anterior duct and excess fat, and fix in 45% acetic acid for 2-5 minutes.
Staining and pressing:
Transfer the gland to lactic acid-acetic acid-orcein staining solution and stain for 5 minutes (avoid evaporation of the staining solution).
Transfer to a slide containing 1:2:3 fixative, cover with a coverslip, tap the coverslip with a dissecting needle to spread the chromosomes, and then press lightly with absorbent paper for blotting (to prevent the coverslip from shifting).
Seal and observe:
Temporary seal: seal the edges of the coverslip with nail polish; permanent seal: peel off the siliconized coverslip after freezing with dry ice-ethanol, dehydrate with 95% and 100% ethanol in turn, and seal with Permount.
Microscope observation: identify chromosome arms (such as X, 2L, 2R, 3L, 3R) by telomere morphology and banding characteristics (such as puff, constriction area).
3. Precautions
45% acetic acid fixation can reduce chromosome puffing, which is better than physiological saline dissection.
Avoid excessive force when pressing the slice to cause chromosome breakage. Light spiral or zigzag pressure can promote uniform spreading.
Different fruit fly strains and culture conditions may lead to differences in banding patterns, which need to be combined with standard pattern analysis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Orcein Related Antibodies
In Vivo

In the apoE/LDLR-/- mouse brachiocephalic atherosclerotic plaque model, Orcein (1 g/100 mL; 20 min, 56°C) can stain elastic fibers purple, collagen fibers blue, red blood cells yellow, and mature fibrin red when combined with Martius Scarlet Blue (MSB) (OMSB), thereby achieving qualitative and quantitative analysis of plaque components[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
CAS No.
Appearance

Solid

Color

Reddish brown to black

SMILES

[Orcein]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Ethanol : < 1 mg/mL (insoluble)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL; Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

SDS (392 KB)

COA (247 KB)

Handling Instructions (2659 KB)
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Orcein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Orcein1400-62-0Biochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Orcein
Cat. No.:
HY-122266
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (392 KB) Français - FR (392 KB) Deutsch - DE (392 KB) Norwegian - NO (392 KB) Español - ES (392 KB) Swedish - SV (392 KB) Italian - IT (392 KB) Portuguese - PT (392 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.