Nα,Nα-Bis(carboxymethyl)-L-lysine

Name: Nα,Nα-Bis(carboxymethyl)-L-lysine
Brand: MedChemExpress
SKU: HY-100047
Rating: 4.5 (1 reviews)

Cat. No.: HY-100047 Purity: 98.80%: Data Sheet
COA

SDS
Handling Instructions
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Nα,Nα-Bis(carboxymethyl)-L-lysine is a competitive inhibitor of bitter taste receptor 4, with an IC₅₀ of 59 nM. Nα,Nα-Bis(carboxymethyl)-L-lysine can be used in bitter receptors related study.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Nα,Nα-Bis(carboxymethyl)-L-lysine Chemical Structure

CAS No. : 113231-05-3

Size	Stock	Quantity
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Nα,Nα-Bis(carboxymethyl)-L-lysine (59 nM) decreases the basal activity of H214A (constitutively active mutant of T2R4) by 40%^[1].
Nα,Nα-Bis(carboxymethyl)-L-lysine (60 nM, 15 mins) blocks the decrease of basal activation state of Rac1 caused by quinine in HEK293T cells overexpressing T2R4 and Ga16/44, but has no effect in HEK293T control cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Nα,Nα-Bis(carboxymethyl)-L-lysine (10 μM) has no effect on lick responses to bitter compounds in adult C57BL6/J^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

262.26

Formula

C₁₀H₁₈N₂O₆

CAS No.

113231-05-3

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 25 mg/mL (95.33 mM)

DMSO : 1.82 mg/mL (6.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : < 1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8130 mL	19.0650 mL	38.1301 mL
5 mM	0.7626 mL	3.8130 mL	7.6260 mL
10 mM	0.3813 mL	1.9065 mL	3.8130 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.80%

Select Batch:

Data Sheet (273 KB) SDS (394 KB)

COA (247 KB) HNMR (510 KB) RP-HPLC (247 KB) MS (82 KB)

Handling Instructions (2659 KB)

References

[1]. Pydi SP, et al. Amino acid derivatives as bitter taste receptor (T2R) blockers. J Biol Chem. 2014;289(36):25054-25066. [Content Brief]

[2]. Sidhu C, et al. Regulation of Rac1 GTPase activity by quinine through G-protein and bitter taste receptor T2R4. Mol Cell Biochem. 2017;426(1-2):129-136. [Content Brief]

[3]. Masamoto M, et al. Yoshida R. Effects of bitter receptor antagonists on behavioral lick responses of mice. Neurosci Lett. 2020;730:135041. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.8130 mL	19.0650 mL	38.1301 mL	95.3252 mL
DMSO / H₂O	5 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
H₂O	10 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	15 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL
	20 mM	0.1907 mL	0.9533 mL	1.9065 mL	4.7663 mL
	25 mM	0.1525 mL	0.7626 mL	1.5252 mL	3.8130 mL
	30 mM	0.1271 mL	0.6355 mL	1.2710 mL	3.1775 mL
	40 mM	0.0953 mL	0.4766 mL	0.9533 mL	2.3831 mL
	50 mM	0.0763 mL	0.3813 mL	0.7626 mL	1.9065 mL
	60 mM	0.0636 mL	0.3178 mL	0.6355 mL	1.5888 mL
	80 mM	0.0477 mL	0.2383 mL	0.4766 mL	1.1916 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.