2. Epigenetics Apoptosis
  3. Histone Acetyltransferase Apoptosis
  4. NU9056

NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells.

For research use only. We do not sell to patients.

NU9056

NU9056 Chemical Structure

CAS No. : 1450644-28-6

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Liquid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

NU9056 Related Antibodies

Top Publications Citing Use of Products

View All Histone Acetyltransferase Isoform Specific Products:

View All Isoforms
CBP/p300 GCN5/PCAF TIP60 MOZ/MORF HBO1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an [1][2] of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells[1].

In Vitro

NU9056 (17-36 μM; 24-96 hours) results in both caspase 3 and caspase 9 activation in a time- and concentration-dependent manner[1].
? NU9056 (2.5-40 μM; 2 hours) treatment results in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation[1].
? NU9056 treatment also decreases androgen receptor, prostate specific antigen, p53 and p21 protein levels[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NU9056 Related Antibodies

Apoptosis Analysis[1]

Cell Line: LNCaP cells
Concentration: 17 µM, 24 µM, 36 µM
Incubation Time: 24 hours, 48 hours, 72 hours, 96 hours
Result: Induced apoptosis via activation of caspase 3 and caspase 9 in a concentration- and time-dependent manner.

Western Blot Analysis[1]

Cell Line: LNCaP cells
Concentration: 2.5 µM, 5 µM, 10 µM, 20 µM, 40 µM
Incubation Time: 2 hours
Result: Resulted in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation.
In Vivo

The mice are injected with Nu9056 (2 μg/g) and the hippocampus is collected 1 h later. Tip60 inhibition reduces H2A.Z binding at the -1 nucleosome of Arc, and the +1 nucleosome of Arc and Syp. Additionally, Nu9056 increases acetylation at the -1 nucleosome of Fos, Tacstd2, and Gria4, and the +1 nucleosome of Gria4[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

232.37

Formula

C6H4N2S4
CAS No.
Appearance

Liquid (Density: 1.60±0.1 g/cm3)

Color

Yellow to brown

SMILES

C1(SSC2=CC=NS2)=CC=NS1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (537.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3035 mL 21.5174 mL 43.0348 mL
5 mM 0.8607 mL 4.3035 mL 8.6070 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.81%

SDS (252 KB)

COA (250 KB) HNMR (231 KB) LCMS (271 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.3035 mL 21.5174 mL 43.0348 mL 107.5870 mL
5 mM 0.8607 mL 4.3035 mL 8.6070 mL 21.5174 mL
10 mM 0.4303 mL 2.1517 mL 4.3035 mL 10.7587 mL
15 mM 0.2869 mL 1.4345 mL 2.8690 mL 7.1725 mL
20 mM 0.2152 mL 1.0759 mL 2.1517 mL 5.3794 mL
25 mM 0.1721 mL 0.8607 mL 1.7214 mL 4.3035 mL
30 mM 0.1434 mL 0.7172 mL 1.4345 mL 3.5862 mL
40 mM 0.1076 mL 0.5379 mL 1.0759 mL 2.6897 mL
50 mM 0.0861 mL 0.4303 mL 0.8607 mL 2.1517 mL
60 mM 0.0717 mL 0.3586 mL 0.7172 mL 1.7931 mL
80 mM 0.0538 mL 0.2690 mL 0.5379 mL 1.3448 mL
100 mM 0.0430 mL 0.2152 mL 0.4303 mL 1.0759 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

NU9056 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NU90561450644-28-6NU 9056NU-9056Histone AcetyltransferaseApoptosisHATsHATTip60KAT5apoptosisprostatecanceracetylationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
NU9056
Cat. No.:
HY-110127
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.