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  3. NOBA

NOBA is a synthetic chromogenic substrate that can be used to detect the enzyme activity of AplTX-II. NOBA can be used in the research of phospholipid hydrolysis.

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NOBA

NOBA Chemical Structure

CAS No. : 55894-52-5

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Description

NOBA is a synthetic chromogenic substrate that can be used to detect the enzyme activity of AplTX-II. NOBA can be used in the research of phospholipid hydrolysis[1].

Molecular Weight

309.31

Formula

C15H19NO6

CAS No.
Appearance

Solid

Color

off-white

SMILES

O=C(O)C1=CC=C([N+]([O-])=O)C(OC(CCCCCCC)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (323.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2330 mL 16.1650 mL 32.3300 mL
5 mM 0.6466 mL 3.2330 mL 6.4660 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2330 mL 16.1650 mL 32.3300 mL 80.8251 mL
5 mM 0.6466 mL 3.2330 mL 6.4660 mL 16.1650 mL
10 mM 0.3233 mL 1.6165 mL 3.2330 mL 8.0825 mL
15 mM 0.2155 mL 1.0777 mL 2.1553 mL 5.3883 mL
20 mM 0.1617 mL 0.8083 mL 1.6165 mL 4.0413 mL
25 mM 0.1293 mL 0.6466 mL 1.2932 mL 3.2330 mL
30 mM 0.1078 mL 0.5388 mL 1.0777 mL 2.6942 mL
40 mM 0.0808 mL 0.4041 mL 0.8083 mL 2.0206 mL
50 mM 0.0647 mL 0.3233 mL 0.6466 mL 1.6165 mL
60 mM 0.0539 mL 0.2694 mL 0.5388 mL 1.3471 mL
80 mM 0.0404 mL 0.2021 mL 0.4041 mL 1.0103 mL
100 mM 0.0323 mL 0.1617 mL 0.3233 mL 0.8083 mL
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NOBA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
NOBA
Cat. No.:
HY-137799
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