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Nitrazine yellow  (Synonyms: Nitrazine paper; Phenaphthazine)

Cat. No.: HY-128545 Purity: 99.31%
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Nitrazine yellow (Nitrazine paper; Phenaphthazine) is a pH indicator. The color of Nitrazine yellow changes from yellow to blue in the pH range from 6.0 to 7.0. Nitrazine yellow is a kind of biological materials or organic compounds that are widely used in life science research.

For research use only. We do not sell to patients.

Nitrazine yellow

Nitrazine yellow Chemical Structure

CAS No. : 5423-07-4

Size Price Stock Quantity
1 g In-stock
5 g   Get quote  
10 g   Get quote  

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Based on 1 publication(s) in Google Scholar

Nitrazine yellow Related Antibodies

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Description

Nitrazine yellow (Nitrazine paper; Phenaphthazine) is a pH indicator. The color of Nitrazine yellow changes from yellow to blue in the pH range from 6.0 to 7.0. Nitrazine yellow is a kind of biological materials or organic compounds that are widely used in life science research[1].

Molecular Weight

542.36

Formula

C16H8N4Na2O11S2
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=S(C1=C(/N=N/C2=CC=C([N+]([O-])=O)C=C2[N+]([O-])=O)C(O)=C3C=CC(S(=O)(O[Na])=O)=CC3=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (92.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (18.44 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8438 mL 9.2190 mL 18.4379 mL
5 mM 0.3688 mL 1.8438 mL 3.6876 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.8438 mL 9.2190 mL 18.4379 mL 46.0948 mL
5 mM 0.3688 mL 1.8438 mL 3.6876 mL 9.2190 mL
10 mM 0.1844 mL 0.9219 mL 1.8438 mL 4.6095 mL
15 mM 0.1229 mL 0.6146 mL 1.2292 mL 3.0730 mL
DMSO 20 mM 0.0922 mL 0.4609 mL 0.9219 mL 2.3047 mL
25 mM 0.0738 mL 0.3688 mL 0.7375 mL 1.8438 mL
30 mM 0.0615 mL 0.3073 mL 0.6146 mL 1.5365 mL
40 mM 0.0461 mL 0.2305 mL 0.4609 mL 1.1524 mL
50 mM 0.0369 mL 0.1844 mL 0.3688 mL 0.9219 mL
60 mM 0.0307 mL 0.1536 mL 0.3073 mL 0.7682 mL
80 mM 0.0230 mL 0.1152 mL 0.2305 mL 0.5762 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Nitrazine yellow Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitrazine yellow5423-07-4Nitrazine paper PhenaphthazineBiochemical Assay Reagentsacid-base indicatorInhibitorinhibitorinhibit

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