2. Cytoskeleton
  3. Collagen
  4. Neutral protease I

Neutral protease I (Dispase I) is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. Neutral protease I can also separate intact epithelial sheets in culture from the substratum. Neutral protease I preserves the viability of the epithelial cells while cleaving the basement membrane zone region. Neutral protease I can also be used to prevent clumping in suspension cultures. Neutral protease I cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.

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Neutral protease I Chemical Structure

Neutral protease I Chemical Structure

CAS No. : 42613-33-2

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Based on 1 publication(s) in Google Scholar

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Neutral protease I Related Antibodies

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Description

Neutral protease I (Dispase I) is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. Neutral protease I can also separate intact epithelial sheets in culture from the substratum. Neutral protease I preserves the viability of the epithelial cells while cleaving the basement membrane zone region. Neutral protease I can also be used to prevent clumping in suspension cultures. Neutral protease I cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin[1].

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Neutral protease I]
Enzyme Activity

≥10 U/mg soild
Unit Definition

One unit is defined as the amount of enzyme that can hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (251 KB)

COA (243 KB)

Handling Instructions (2659 KB)
References
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Neutral protease I Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neutral protease I42613-33-2Dispase ICollagenEDTA、EGTA、Hg2+Inhibitorinhibitorinhibit

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