2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye β-glucuronidase
  4. Naphthol AS-BI

Naphthol AS-BI is a substrate of β-glucuronidase and produces a bright red effect after staining biological tissues.

For research use only. We do not sell to patients.

Naphthol AS-BI Chemical Structure

Naphthol AS-BI Chemical Structure

CAS No. : 1237-75-8

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Based on 1 publication(s) in Google Scholar

Naphthol AS-BI Related Antibodies

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Description

Naphthol AS-BI is a substrate of β-glucuronidase and produces a bright red effect after staining biological tissues.

Molecular Weight

372.21

Formula

C18H14BrNO3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(O)C=C2C=CC(Br)=CC2=C1)NC3=C(OC)C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (134.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6867 mL 13.4333 mL 26.8666 mL
5 mM 0.5373 mL 2.6867 mL 5.3733 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6867 mL 13.4333 mL 26.8666 mL 67.1664 mL
5 mM 0.5373 mL 2.6867 mL 5.3733 mL 13.4333 mL
10 mM 0.2687 mL 1.3433 mL 2.6867 mL 6.7166 mL
15 mM 0.1791 mL 0.8956 mL 1.7911 mL 4.4778 mL
20 mM 0.1343 mL 0.6717 mL 1.3433 mL 3.3583 mL
25 mM 0.1075 mL 0.5373 mL 1.0747 mL 2.6867 mL
30 mM 0.0896 mL 0.4478 mL 0.8956 mL 2.2389 mL
40 mM 0.0672 mL 0.3358 mL 0.6717 mL 1.6792 mL
50 mM 0.0537 mL 0.2687 mL 0.5373 mL 1.3433 mL
60 mM 0.0448 mL 0.2239 mL 0.4478 mL 1.1194 mL
80 mM 0.0336 mL 0.1679 mL 0.3358 mL 0.8396 mL
100 mM 0.0269 mL 0.1343 mL 0.2687 mL 0.6717 mL
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Naphthol AS-BI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naphthol AS-BI1237-75-8Fluorescent Dyeβ-glucuronidaseGUSBInhibitorinhibitorinhibit

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