N6-Methyladenine

Cat. No.: HY-116887 Purity: 98.79%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

N6-Methyladenine is a DNA epigenetic modification that involves the addition of a methyl group to the sixth position of adenine. N6-Methyladenine plays an important role in distinguishing host DNA from exogenous DNA and controls many biological functions, such as DNA replication, transcription, mismatch repair, and chromosome replication. N6-Methyladenine can be used for the kidney diseases.

For research use only. We do not sell to patients.

N6-Methyladenine Chemical Structure

CAS No. : 443-72-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of N6-Methyladenine:

N6-Methyladenine (Standard) Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

N6-methyladenine is the most common internal epigenetic modification in eukaryotic mRNA and regulates its dynamic and reversible process through three classes of enzymes: methyltransferases (writers), demethylases (erasers), and methylation recognition proteins (readers). N6-methyladenine plays a central role in key biological processes such as RNA splicing, stability, translocation, and translation. N6-methyladenine is involved in physiological processes such as nervous system development. N6-methyladenine affects the pathogenesis of acute kidney injury (AKI, such as ischemia-reperfusion-induced AKI, cisplatin-induced AKI), acute kidney disease (AKD), and chronic kidney disease (CKD, such as diabetic kidney disease, lupus nephritis, autosomal dominant polycystic kidney disease) by regulating target genes (e.g., Foxd1, YAP1, TAB3, Sirt1) and related signaling pathways (e.g., PI3K/Akt, ERK/NF-κB, NLRP3 inflammasome)^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

149.16

Formula

C₆H₇N₅

CAS No.

443-72-1

Appearance

Solid

Color

White to light brown

SMILES

CNC1=C2NC=NC2=NC=N1

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (134.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 2 mg/mL (13.41 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7042 mL	33.5211 mL	67.0421 mL
5 mM	1.3408 mL	6.7042 mL	13.4084 mL
10 mM	0.6704 mL	3.3521 mL	6.7042 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (13.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (13.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (13.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (278 KB) SDS (476 KB)

COA (255 KB) HNMR (131 KB) RP-HPLC (238 KB) MS (298 KB)

Handling Instructions (2659 KB)

References

[1]. Hao Li, et al. DNA N6-Methyladenine Modification in Eukaryotic Genome. Front Genet. 2022 Jun 24:13:914404. [Content Brief]

[2]. Luan J, et al. N6-methyladenine RNA Methylation Epigenetic Modification and Kidney Diseases. Kidney Int Rep. 2022 Oct 17;8(1):36-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	6.7042 mL	33.5211 mL	67.0421 mL	167.6053 mL
	5 mM	1.3408 mL	6.7042 mL	13.4084 mL	33.5211 mL
	10 mM	0.6704 mL	3.3521 mL	6.7042 mL	16.7605 mL
DMSO	15 mM	0.4469 mL	2.2347 mL	4.4695 mL	11.1737 mL
	20 mM	0.3352 mL	1.6761 mL	3.3521 mL	8.3803 mL
	25 mM	0.2682 mL	1.3408 mL	2.6817 mL	6.7042 mL
	30 mM	0.2235 mL	1.1174 mL	2.2347 mL	5.5868 mL
	40 mM	0.1676 mL	0.8380 mL	1.6761 mL	4.1901 mL
	50 mM	0.1341 mL	0.6704 mL	1.3408 mL	3.3521 mL
	60 mM	0.1117 mL	0.5587 mL	1.1174 mL	2.7934 mL
	80 mM	0.0838 mL	0.4190 mL	0.8380 mL	2.0951 mL
	100 mM	0.0670 mL	0.3352 mL	0.6704 mL	1.6761 mL