N-Biotinyl-L-cysteine

Cat. No.: HY-128466 Purity: ≥95.0%: Data Sheet
COA

SDS
Handling Instructions
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N-Biotinyl-L-cysteine is a biotinylated biochemical assay reagent, which is used to detect avidin and biotin through a competitive binding reaction.

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N-Biotinyl-L-cysteine Chemical Structure

CAS No. : 151009-85-7

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Purity & Documentation
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Description

N-Biotinyl-L-cysteine is a biotinylated biochemical assay reagent, which is used to detect avidin and biotin through a competitive binding reaction^[1].

Molecular Weight

347.45

Formula

C₁₃H₂₁N₃O₄S₂

CAS No.

151009-85-7

Appearance

Solid

Color

White to off-white

SMILES

SC[C@@H](C(O)=O)NC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (71.95 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8781 mL	14.3906 mL	28.7811 mL
5 mM	0.5756 mL	2.8781 mL	5.7562 mL
10 mM	0.2878 mL	1.4391 mL	2.8781 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥95.0%

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Data Sheet (267 KB) SDS (251 KB)

COA (247 KB) HNMR (268 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Sugawara K, et al., Voltammetric detection of avidin and biotin by biotin labeled with cysteine[J]. Fresenius' journal of analytical chemistry, 1997, 358: 755-759.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8781 mL	14.3906 mL	28.7811 mL	71.9528 mL
	5 mM	0.5756 mL	2.8781 mL	5.7562 mL	14.3906 mL
	10 mM	0.2878 mL	1.4391 mL	2.8781 mL	7.1953 mL
	15 mM	0.1919 mL	0.9594 mL	1.9187 mL	4.7969 mL
	20 mM	0.1439 mL	0.7195 mL	1.4391 mL	3.5976 mL
	25 mM	0.1151 mL	0.5756 mL	1.1512 mL	2.8781 mL
	30 mM	0.0959 mL	0.4797 mL	0.9594 mL	2.3984 mL
	40 mM	0.0720 mL	0.3598 mL	0.7195 mL	1.7988 mL
	50 mM	0.0576 mL	0.2878 mL	0.5756 mL	1.4391 mL
	60 mM	0.0480 mL	0.2398 mL	0.4797 mL	1.1992 mL