Myoseverin

Name: Myoseverin
Brand: MedChemExpress
SKU: HY-W008956
Rating: 4.5 (1 reviews)

Cat. No.: HY-W008956 Purity: 99.42%: Data Sheet
COA

SDS
Handling Instructions
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Myoseverin is a microtubule-binding molecule and angiogenesis inhibitor. Myoseverin can induce the reversible fission of multinucleated myotubes into mononucleated fragments. In addition, Myoseverin exerts anti-angiogenic effects by inhibiting endothelial cell function and endothelial progenitor cell differentiation.

For research use only. We do not sell to patients.

Myoseverin Chemical Structure

CAS No. : 267402-71-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: Myoseverin	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 31610375]
CCRF-CEM	IC₅₀	5.7 μM Compound: myoseverin	Cytotoxicity against human CEM cells after 72 hrs by MTT assay Cytotoxicity against human CEM cells after 72 hrs by MTT assay	[PMID: 19349183]
HCT-116	IC₅₀	3.3 μM Compound: myoseverin	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 19349183]
HCT-116	IC₅₀	7.82 μM Compound: Myoseverin	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 31610375]
HepG2	IC₅₀	3.06 μM Compound: Myoseverin	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 31610375]
MCF7	IC₅₀	11.5 μM Compound: myoseverin	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 19349183]
MES-SA	IC₅₀	8.5 μM Compound: myoseverin	Cytotoxicity against human MES-SA cells after 72 hrs by MTT assay Cytotoxicity against human MES-SA cells after 72 hrs by MTT assay	[PMID: 19349183]
SW48	IC₅₀	2.7 μM Compound: myoseverin	Cytotoxicity against human SW48 cells after 72 hrs by MTT assay Cytotoxicity against human SW48 cells after 72 hrs by MTT assay	[PMID: 19349183]
SW480	IC₅₀	4.8 μM Compound: myoseverin	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 19349183]

In Vitro

Myoseverin (20 μM; 24 h) promotes the fission of multinucleated myotubes into mononucleated fragments with "bead-on-a-string" constriction morphology, and causes disassembly of the microtubule cytoskeleton with microtubules aggregating around the nucleus and fragmenting in mouse C2C12 myotubes^[1].
Myoseverin (20 μM; 24 h) promotes DNA synthesis and colony formation, and regulates the expression of more than 93 genes, involving extracellular matrix remodeling, growth factors, immunomodulatory pathways, etc in mouse C2C12 myotubes^[1].
Myoseverin (0-80 μM; 1-7 d) inhibits cell proliferation (IC₅₀: 8 μM) in a reversible manner and suppresses cell migration in human umbilical vein endothelial cells^[2].
Myoseverin (0-80 μM; 7 d) reduces the differentiation of endothelial progenitor cells into adherent cells in human cord blood mononuclear cells^[2].
Myoseverin (0-20 μM; 7 d) inhibits the acetylated low-density lipoprotein (ac-LDL) uptake ability and the expression of endothelial markers (KDR, CD31, vWF) in endothelial progenitor cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Human umbilical vein endothelial cells
Concentration:	0, 2.5, 5, 10, 20, 40 and 80 μM
Incubation Time:	72 h
Result:	Inhibited the cell viability.

In Vivo

Myoseverin (0-10 μg; topical application; single dose) inhibits in vivo angiogenesis in the chick chorioallantoic membrane model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4.5-day-old chick embryos (chorioallantoic membrane (CAM) model was established by normal incubation of eggs)^[2]
Dosage:	0, 0.1, 1, 5 and 10 μg/embryo
Administration:	Topical application; single dose (48 h)
Result:	Did not show significant inhibition at low doses (0.1 μg or 1 μg/embryo). Potently inhibited in vivo angiogenesis by showing a marked inhibition zone around the implanted cover slip without affecting embryo’s viability at 5 μg or 10 μg/embryo.

Molecular Weight

432.52

Formula

C₂₄H₂₈N₆O₂

CAS No.

267402-71-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC(N1C=NC2=C(NCC3=CC=C(OC)C=C3)N=C(NCC4=CC=C(OC)C=C4)N=C12)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (578.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3120 mL	11.5602 mL	23.1203 mL
5 mM	0.4624 mL	2.3120 mL	4.6241 mL
10 mM	0.2312 mL	1.1560 mL	2.3120 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (4.81 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.81 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Rosania GR, et al. Myoseverin, a microtubule-binding molecule with novel cellular effects. Nat Biotechnol. 2000;18(3):304-308. [Content Brief]

[2]. Park HE, et al. Myoseverin is a potential angiogenesis inhibitor by inhibiting endothelial cell function and endothelial progenitor cell differentiation. DNA Cell Biol. 2006 Sep;25(9):514-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3120 mL	11.5602 mL	23.1203 mL	57.8008 mL
	5 mM	0.4624 mL	2.3120 mL	4.6241 mL	11.5602 mL
	10 mM	0.2312 mL	1.1560 mL	2.3120 mL	5.7801 mL
	15 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8534 mL
	20 mM	0.1156 mL	0.5780 mL	1.1560 mL	2.8900 mL
	25 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3120 mL
	30 mM	0.0771 mL	0.3853 mL	0.7707 mL	1.9267 mL
	40 mM	0.0578 mL	0.2890 mL	0.5780 mL	1.4450 mL
	50 mM	0.0462 mL	0.2312 mL	0.4624 mL	1.1560 mL
	60 mM	0.0385 mL	0.1927 mL	0.3853 mL	0.9633 mL
	80 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7225 mL
	100 mM	0.0231 mL	0.1156 mL	0.2312 mL	0.5780 mL

Myoseverin Related Classifications

Inflammation/Immunology Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Myoseverin267402-71-1Microtubule/TubulinDNA/RNA Synthesispurinemicrotubuleswoundhealingtissueregenerationmononucleatedfragmentschorioallantoic membrane model C2C12 myotubeshuman umbilical vein endothelial cellsendothelial progenitor cellsInhibitorinhibitorinhibit

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Myoseverin

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