Methyltetrazine-Maleimide

Name: Methyltetrazine-Maleimide
Brand: MedChemExpress
SKU: HY-136104
Rating: 4.5 (1 reviews)

Cat. No.: HY-136104 Purity: 97.97%: Data Sheet
COA

SDS
Handling Instructions
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Methyltetrazine-Maleimide is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Methyltetrazine-Maleimide is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

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Methyltetrazine-Maleimide Chemical Structure

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Small Molecules:

•Related Proteins:

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View All Isoforms

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Methyltetrazine-Maleimide is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. Methyltetrazine-Maleimide is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Cleavable Linker

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

352.35

Formula

C₁₇H₁₆N₆O₃

Appearance

Solid

Color

Dark purple to black

SMILES

CC(N=N1)=NN=C1C2=CC=C(CNC(CCN3C(C=CC3=O)=O)=O)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (283.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8381 mL	14.1904 mL	28.3809 mL
5 mM	0.5676 mL	2.8381 mL	5.6762 mL
10 mM	0.2838 mL	1.4190 mL	2.8381 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.97%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (247 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8381 mL	14.1904 mL	28.3809 mL	70.9522 mL
	5 mM	0.5676 mL	2.8381 mL	5.6762 mL	14.1904 mL
	10 mM	0.2838 mL	1.4190 mL	2.8381 mL	7.0952 mL
	15 mM	0.1892 mL	0.9460 mL	1.8921 mL	4.7301 mL
	20 mM	0.1419 mL	0.7095 mL	1.4190 mL	3.5476 mL
	25 mM	0.1135 mL	0.5676 mL	1.1352 mL	2.8381 mL
	30 mM	0.0946 mL	0.4730 mL	0.9460 mL	2.3651 mL
	40 mM	0.0710 mL	0.3548 mL	0.7095 mL	1.7738 mL
	50 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4190 mL
	60 mM	0.0473 mL	0.2365 mL	0.4730 mL	1.1825 mL
	80 mM	0.0355 mL	0.1774 mL	0.3548 mL	0.8869 mL
	100 mM	0.0284 mL	0.1419 mL	0.2838 mL	0.7095 mL