Methyl jasmonate

Cat. No.: HY-135663: Data Sheet Handling Instructions
FAQs
Technical Support

Methyl jasmonate is a phytohormone that regulates the defense response of plants under biotic and biotic stress through jasmonate signaling pathway. Methyl jasmonate inhibits the activation of NF-κB signaling pathway. Methyl jasmonate can promote the mitochondrial ROS production, but also scavenges free radicals and reduces the oxidative stress. Methyl jasmonate exhibits anti-inflammatory, antitumor, anticonvulsant, antinociceptive and sedative activities.

For research use only. We do not sell to patients.

Methyl jasmonate Chemical Structure

CAS No. : 1211-29-6

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of Methyl jasmonate:

(rac)-Methyl jasmonate In-stock
(rac)-Methyl jasmonate (Standard) Get quote

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Phytohormone Isoform Specific Products:

View All Isoforms

Gibberellins

View All NF-κB Isoform Specific Products:

View All Isoforms

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Methyl jasmonate (1-2 mM, 24 h-14 days) suppresses the expression of PCNA, XIAP and survivin, inhibits the cell viability (IC₅₀s for SK-N-SH and BE(2)-C are 1.39 mM and 1.35 mM) and colony formation of human neuroblastoma cells SK-N-SH and BE(2)-C, induces cell arrest at G0/G1 phase, and induces apoptosis in SK-N-SH and BE(2)-C cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	SK-N-SH and BE(2)-C
Concentration:	1-2 mM
Incubation Time:	24 h
Result:	Inhibited the cell viability.

Apoptosis Analysis^[3]

Cell Line:	SK-N-SH and BE(2)-C
Concentration:	1-2 mM
Incubation Time:	24 h
Result:	Induced apoptosis.

In Vivo

Methyl jasmonate (75-300 mg/kg, po, once daily for 18 days) reduce inflammatory response and oxidative stress in Freund’s adjuvant (HY-153808)-induced rats arthritis models^[1].
Methyl jasmonate (10-40 mg/kg, ip, once daily for 7 days) inhibits the AChE activity in mice brains, enhances the spatial working memory of mice, and reverses Scopolamine (HY-N0296)-induced memory impairment in Swiss mouse models^[5].
Methyl jasmonate (10-50 mg/kg, ip, single dose) exhibits anti-inflammatory and analgesic activities in Acetic acid (HY-Y0319)-induced mouse writhing models^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Freund’s adjuvant (HY-153808)-induced rats arthritis models^[1]
Dosage:	75-300 mg/kg
Administration:	po, once daily for 18 days
Result:	Reduced inflammatory markers in the brains of arthritic rats, such as MPO activity and NO levels. Increased levels of oxidative stress markers, such as nitrates, nitrites, lipid peroxides and ROS. Increased the level of reduced glutathione (GSH) in rats brains

Animal Model:	Scopolamine (HY-N0296)-induced memory impairment in Swiss mouse models^[5]
Dosage:	10-40 mg/kg
Administration:	ip, once daily for 7 days
Result:	Increased the alternation behavior and preference index in the Y-maze and ORT tests.

Animal Model:	Acetic acid (HY-Y0319)-induced mouse writhing models^[6]
Dosage:	10-50 mg/kg
Administration:	ip, single dose
Result:	Reduced the number of writhing.

Molecular Weight

224.30

Formula

C₁₃H₂₀O₃

CAS No.

1211-29-6

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(OC)C[C@@H]1[C@@H](C/C=C\CC)C(CC1)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (445.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4583 mL	22.2916 mL	44.5831 mL
5 mM	0.8917 mL	4.4583 mL	8.9166 mL
10 mM	0.4458 mL	2.2292 mL	4.4583 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Pereira-Maróstica HV, et al., Methyl Jasmonate Reduces Inflammation and Oxidative Stress in the Brain of Arthritic Rats. Antioxidants (Basel). 2019 Oct 15;8(10):485. [Content Brief]

[2]. Yu X, et al., The roles of methyl jasmonate to stress in plants. Funct Plant Biol. 2019 Feb;46(3):197-212. [Content Brief]

[3]. Tong QS, et al., Methyl jasmonate downregulates expression of proliferating cell nuclear antigen and induces apoptosis in human neuroblastoma cell lines. Anticancer Drugs. 2008 Jul;19(6):573-81. [Content Brief]

[4]. Annafi OS, et al., Evaluation of the anticonvulsant and anxiolytic potentials of methyl jasmonate in mice. Sci Pharm. 2014 Mar 24;82(3):643-54. [Content Brief]

[5]. Eduviere AT, et al., Methyl jasmonate enhances memory performance through inhibition of oxidative stress and acetylcholinesterase activity in mice. Life Sci. 2015 Jul 1;132:20-6. [Content Brief]

[6]. Umukoro S, et al., Antinociceptive effects of methyl jasmonate in experimental animals. J Nat Med. 2011 Jul;65(3-4):466-70. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4583 mL	22.2916 mL	44.5831 mL	111.4579 mL
	5 mM	0.8917 mL	4.4583 mL	8.9166 mL	22.2916 mL
	10 mM	0.4458 mL	2.2292 mL	4.4583 mL	11.1458 mL
	15 mM	0.2972 mL	1.4861 mL	2.9722 mL	7.4305 mL
	20 mM	0.2229 mL	1.1146 mL	2.2292 mL	5.5729 mL
	25 mM	0.1783 mL	0.8917 mL	1.7833 mL	4.4583 mL
	30 mM	0.1486 mL	0.7431 mL	1.4861 mL	3.7153 mL
	40 mM	0.1115 mL	0.5573 mL	1.1146 mL	2.7864 mL
	50 mM	0.0892 mL	0.4458 mL	0.8917 mL	2.2292 mL
	60 mM	0.0743 mL	0.3715 mL	0.7431 mL	1.8576 mL
	80 mM	0.0557 mL	0.2786 mL	0.5573 mL	1.3932 mL
	100 mM	0.0446 mL	0.2229 mL	0.4458 mL	1.1146 mL