Methoxyacetic acid

Name: Methoxyacetic acid
Brand: MedChemExpress
SKU: HY-Y1009
Rating: 4.5 (1 reviews)

Cat. No.: HY-Y1009 Purity: 99.64%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

Methoxyacetic acid is a metabolite of ethylene glycol monomethyl ether. When the concentration of methoxyacetic acid reaches a certain level, it can inhibit the respiratory function of hepatic mitochondria and testicular mitochondria. Methoxyacetic acid is somewhat toxic.

For research use only. We do not sell to patients.

Methoxyacetic acid Chemical Structure

CAS No. : 625-45-6

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Methoxyacetic acid:

Methoxyacetic acid-d₃ Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Methoxyacetic acid (>5 mM) inhibits the state 3 succinate oxidation rate and the respiratory control ratio in hepatic mitochondria^[1].
Methoxyacetic acid (>3 mM) inhibits the ascorbate/TMPD oxidation rate in testicular mitochondria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Methoxyacetic acid (30-300 mg/kg; oral gavage; 8 times, dosed over 10 days) is somewhat toxic to male Fischer 344 rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Fischer 344 rats (6-8 weeks of age)^[2]
Dosage:	30, 100 and 300 mg/kg
Administration:	Oral gavage (i.g.); 5 consecutive days of the first week followed by 3 more consecutive daily doses after a weekend interruption.
Result:	Led to a significant decrease in body weight compared to the control group. It also caused severe degeneration of testicular germinal epithelium, a marked reduction in the size of the thymus with depletion of thymic cortical lymphoid elements, and a reduction in bone marrow cellularity, resulting in lower red blood cell counts, hemoglobin concentration, packed cell volume, and white blood cell counts at dose of 300 mg/kg. Had some similar but less pronounced effects, such as a significant decrease in the relative thymus weight and slight degenerative changes in testicular germinal epithelium in some rats at dose of 100 mg/kg. Produced no apparent effects during the course of the study at dose of 30 mg/kg.

Molecular Weight

90.08

Formula

C₃H₆O₃

CAS No.

625-45-6

Appearance

Liquid (Density: 1.174 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(O)COC

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (1110.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (1110.12 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	11.1012 mL	55.5062 mL	111.0124 mL
5 mM	2.2202 mL	11.1012 mL	22.2025 mL
10 mM	1.1101 mL	5.5506 mL	11.1012 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (271 KB) SDS (779 KB)

COA (244 KB) HNMR (109 KB) GC (202 KB)

Handling Instructions (2659 KB)

References

[1]. Beattie PJ, et al. Methoxyacetic acid and ethoxyacetic acid inhibit mitochondrial function in vitro. J Biochem Toxicol. 1986 Sep;1(3):61-70. [Content Brief]

[2]. Miller RR, et al. Toxicity of methoxyacetic acid in rats. Fundam Appl Toxicol. 1982 Jul-Aug;2(4):158-60. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	11.1012 mL	55.5062 mL	111.0124 mL	277.5311 mL
	5 mM	2.2202 mL	11.1012 mL	22.2025 mL	55.5062 mL
	10 mM	1.1101 mL	5.5506 mL	11.1012 mL	27.7531 mL
	15 mM	0.7401 mL	3.7004 mL	7.4008 mL	18.5021 mL
	20 mM	0.5551 mL	2.7753 mL	5.5506 mL	13.8766 mL
	25 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	30 mM	0.3700 mL	1.8502 mL	3.7004 mL	9.2510 mL
	40 mM	0.2775 mL	1.3877 mL	2.7753 mL	6.9383 mL
	50 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	60 mM	0.1850 mL	0.9251 mL	1.8502 mL	4.6255 mL
	80 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4691 mL
	100 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.