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MES potassium  (Synonyms: 2-Morpholinoethanesulphonic acid potassium)

Cat. No.: HY-D0858E Purity: 99.88%
Handling Instructions Technical Support

MES (2-Morpholinoethanesulphonic acid) potassium is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES potassium is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.

For research use only. We do not sell to patients.

MES potassium Chemical Structure

MES potassium Chemical Structure

CAS No. : 39946-25-3

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of MES potassium:

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  • Biological Activity

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Description

MES (2-Morpholinoethanesulphonic acid) potassium is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES potassium is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies[1][2].

In Vitro

MES potassium is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES potassium changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES potassium shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 ℃ and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

233.33

Formula

C6H12KNO4S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O[K])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, sealed storage, away from moisture and light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (428.58 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2858 mL 21.4289 mL 42.8578 mL
5 mM 0.8572 mL 4.2858 mL 8.5716 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.88%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.2858 mL 21.4289 mL 42.8578 mL 107.1444 mL
5 mM 0.8572 mL 4.2858 mL 8.5716 mL 21.4289 mL
10 mM 0.4286 mL 2.1429 mL 4.2858 mL 10.7144 mL
15 mM 0.2857 mL 1.4286 mL 2.8572 mL 7.1430 mL
20 mM 0.2143 mL 1.0714 mL 2.1429 mL 5.3572 mL
25 mM 0.1714 mL 0.8572 mL 1.7143 mL 4.2858 mL
30 mM 0.1429 mL 0.7143 mL 1.4286 mL 3.5715 mL
40 mM 0.1071 mL 0.5357 mL 1.0714 mL 2.6786 mL
50 mM 0.0857 mL 0.4286 mL 0.8572 mL 2.1429 mL
60 mM 0.0714 mL 0.3571 mL 0.7143 mL 1.7857 mL
80 mM 0.0536 mL 0.2679 mL 0.5357 mL 1.3393 mL
100 mM 0.0429 mL 0.2143 mL 0.4286 mL 1.0714 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MES potassium
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