Neo-enactin, a new antifungal antibiotic potentiating polyene antifungal antibiotics. I. Fermentation, extraction, purification and physico-chemical and biological properties

A new antifungal Antibiotic, named neo-enactin, was isolated from the cultured mycelia of Strain H 829-MY 10, identified as a new subspecies of Streptoverticillium and named Streptoverticillium olivoreticuli subsp. neoenacticus. The Antibiotic was produced with a tetraene antifungal Antibiotic, found mainly in the cultured mycelia of Strain H 829-MY 10 and it was extracted with methanol. The Antibiotic is of a basic nature and it can be extracted with ethyl acetate at alkaline pH. Purification of neo-enactin was carried out by partition chromatography on cellulose and elution with ethyl acetate bufferized with phosphate buffer (pH 8.0). Neo-enactin shows strong Antifungal activity and potentiates the Antifungal activity of polyene antifungal Antibiotics.