2. Academic Validation
  3. Two-Dimensional FAIMS-TIMS Separation for Probing Structural Diversity of Peptides with Resolution Exceeding 6500

Two-Dimensional FAIMS-TIMS Separation for Probing Structural Diversity of Peptides with Resolution Exceeding 6500

  • Anal Chem. 2025 Nov 4;97(43):24225-24231. doi: 10.1021/acs.analchem.5c05173.
Junhui Li 1 2 3,  Xixuan Gu 1 2 3,  Yongqi Lei 1 2 4,  Kaiqun Wu 1 2 3,  Zhonghan Hu 1 2 3,  Rong Liu 1 2 3,  Chenlu Wang 1 2 3,  Yong Wu 1 2 3,  Jiancheng Yu 1 2 4,  Keqi Tang 1 2 3
Affiliations

Affiliations

  • 1 Institute of Mass Spectrometry, Zhejiang Engineering Research Center of Advanced Mass Spectrometry and Clinical Application, Ningbo University, Ningbo 315211, P. R. China.
  • 2 Zhenhai Institute of Mass Spectrometry, Ningbo 315211, P. R. China.
  • 3 School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, P. R. China.
  • 4 Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo 315211, P. R. China.
PMID: 41116333 DOI: 10.1021/acs.analchem.5c05173
Abstract

A two-dimensional gas-phase ion separation platform was developed by coupling high-sensitivity and high-resolution racetrack field asymmetric waveform ion mobility spectrometry (r-FAIMS) with trapped ion mobility spectrometry (TIMS) and time-of-flight mass spectrometry. This platform was applied to explore the conformational diversity of doubly charged bradykinin (BK+2H+)2+ ions. Four conformers of the (BK+2H+)2+ ion can be resolved by using fully optimized TIMS, while six conformers of the (BK+2H+)2+ ion with different compensation voltages (CV) can be resolved by using r-FAIMS with pure nitrogen as a carrier gas and a 2.6 kV dispersion voltage (DV). By further TIMS analyses of the CV-selected (BK+2H+)2+ ions corresponding to all the six FAIMS separated peaks, nearly 30 conformers of the (BK+2H+)2+ ion can be resolved. Multiple conformers of (BK+2H+)2+ ions were found to have completely different CV values, although their mobilities were essentially the same. Multiple TIMS peaks, which are basically buried in the ambient noise in the normal TIMS spectrum, can be easily detected in the CV-selected TIMS spectra. These experimental measurements have positively proven that the separations in the FAIMS and TIMS dimensions are highly orthogonal. The overall resolving power of two-dimensional r-FAIMS-TIMS separation is essentially proportional to the product of the resolving powers of r-FAIMS and TIMS. The theoretical calculation, based on the data correlation between FAIMS and TIMS measurements, has shown that the total resolving power of the combined r-FAIMS and TIMS separation exceeds 6500 for the (BK+2H+)2+ ions, making the new instrument platform very effective for the structural elucidation of large biomolecules.

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