2. Academic Validation
  3. Epitranscriptomic analysis reveals features of NAD-capped RNAs upon supplementation of nicotinamide mononucleotide in human

Epitranscriptomic analysis reveals features of NAD-capped RNAs upon supplementation of nicotinamide mononucleotide in human

  • Exp Cell Res. 2025 Oct 8;453(1):114780. doi: 10.1016/j.yexcr.2025.114780.
Shuwen Ge 1,  Yandong Liu 2,  Dean Li 1,  Guojing Han 2,  Kongyan Niu 1,  Hujun Ju 3,  Hao Zhou 4,  Rui Liu 5,  Zhengjiang Zhu 6,  Nan Liu 7,  Lefeng Qu 8
Affiliations

Affiliations

  • 1 Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2 Department of Vascular and Endovascular Surgery, Chang Zheng Hospital, Naval Medical University, Shanghai, 200003, China.
  • 3 Redpont Biotechnology, Hongtaiyang Life Science Industrial Park, Dangtu Economic Development Zone, Ma'anshan, Anhui Province, 243000, China.
  • 4 Haven Biotechnology, 1 Jing Ba Rd, Economic Development Zone, Ganzhou, Jiangxi Province, 342499, China.
  • 5 Singlera Genomics, 500 Fu Rong Hua Rd., Pudong, Shanghai, 201204, China.
  • 6 Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; Shanghai Key Laboratory of Aging Studies, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China.
  • 7 Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; Shanghai Key Laboratory of Aging Studies, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China. Electronic address: liunan@sioc.ac.cn.
  • 8 Department of Vascular and Endovascular Surgery, Chang Zheng Hospital, Naval Medical University, Shanghai, 200003, China. Electronic address: qulefeng@smmu.edu.cn.
PMID: 41072840 DOI: 10.1016/j.yexcr.2025.114780
Abstract

Nicotinamide mononucleotide (NMN), a precursory metabolite of NAD, has been demonstrated to boost cellular NAD level that is coupled with various age-related beneficial effects in animal models. NAD-capped RNA (NAD-RNA) represents a critical but poorly studied modification at the epitranscriptomic level. Here we examine the impact of NMN supplementation on NAD-RNA in human peripheral blood mononuclear cells (PBMCs). We demonstrated that NMN supplementation increases NAD turnover coupled with a reduction in NAD-capped RNAs in both human and dog, revealing blood-derived NAD-RNAs as potential biomarkers sensitized to NMN exposure.

Keywords

Epitranscriptome; NAD-Capped RNAs; NMN; PBMC.

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