2. Academic Validation
  3. MINPP1 promotes ferroptosis in HBV-related hepatocellular carcinoma by regulating CTSB K33-linked deubiquitination via ZRANB1

MINPP1 promotes ferroptosis in HBV-related hepatocellular carcinoma by regulating CTSB K33-linked deubiquitination via ZRANB1

  • Biol Direct. 2025 Oct 1;20(1):100. doi: 10.1186/s13062-025-00686-z.
Wenbiao Chen 1,  Yang Song 2,  Liyang Wang 2,  Feng Xiong 2,  Shenggang Zhang 2
Affiliations

Affiliations

  • 1 Department of Gastroenterology, Shenzhen People's Hospital, The Second Clinical Medical College, The First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, China. chanwenbiao@sina.com.
  • 2 Department of Gastroenterology, Shenzhen People's Hospital, The Second Clinical Medical College, The First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, China.
PMID: 41035046 DOI: 10.1186/s13062-025-00686-z
Abstract

Background: Hepatocellular carcinoma (HCC) is significantly influenced by hepatitis B virus (HBV) Infection. However, the roles of Ferroptosis and ubiquitination modifications in this context remain poorly understood.

Methods: In this study, we utilized immunoprecipitation, immunofluorescence, and analysis of ubiquitin modifications to explore the regulatory mechanisms of MINPP1 in Ferroptosis and its effects on tumor progression. Further mechanistic studies revealed that ZRANB1 regulates the K33-linked ubiquitination of CTSB. Ultimately, the contribution of the MINPP1-CTSB axis to tumor progression was validated using in vivo experiments.

Results: Our study demonstrates that MINPP1 regulates Ferroptosis in HBV-positive HCC cells via a glycolytic bypass mechanism. Bioinformatics analysis indicates that MINPP1 stabilizes CTSB, thereby participating in the regulation of Ferroptosis. Specifically, MINPP1 modulates K33-linked deubiquitination of CTSB through ZRANB1, which stabilizes CTSB expression and identifies its deubiquitination site. In contrast, the MINPP1-ZRANB1-CTSB axis does not regulate Ferroptosis in HBV-negative HCC cells. However, upon the introduction of HBV into these cells, the MINPP1-ZRANB1-CTSB axis becomes active and promotes Ferroptosis. Finally, in vivo assays showed that MINPP1 regulates tumor progression by regulating K33-linked ubiquitination of CTSB, thereby affecting Ferroptosis levels.

Conclusion: Our research showed outcomes suggest that the MINPP1-ZRANB1-CTSB axis promotes Ferroptosis in HBV-positive HCC cells through glycolysis, emphasizing the function of MINPP1 in mediating Ferroptosis in HBV-related HCC cells via CTSB deubiquitination modification. This provides valuable insights and a foundation for the treatment of HBV-associated HCC.

Keywords

Hepatitis b virus; Hepatocellular carcinoma; Ubiquitination.

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