2. Academic Validation
  3. Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiation

Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiation

  • STAR Protoc. 2025 Sep 26;6(4):104115. doi: 10.1016/j.xpro.2025.104115.
Qiqi Tang 1,  Juan Xu 2,  Xinyu Yang 2,  Hanni Li 2,  Lingdi Wang 3,  Lu Zhu 4
Affiliations

Affiliations

  • 1 Department of Pharmacology, State Key Laboratory of Experimental Hematology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, NHC Key Laboratory of Hormones and Development, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Department of Physiology and Pathophysiology, Tianjin Key Laboratory of Cell Homeostasis and Major Diseases, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: tangqq630@163.com.
  • 2 Department of Pharmacology, State Key Laboratory of Experimental Hematology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, NHC Key Laboratory of Hormones and Development, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
  • 3 Department of Physiology and Pathophysiology, Tianjin Key Laboratory of Cell Homeostasis and Major Diseases, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: wanglingdi@tmu.edu.cn.
  • 4 Department of Pharmacology, State Key Laboratory of Experimental Hematology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, NHC Key Laboratory of Hormones and Development, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: zhulu@tmu.edu.cn.
PMID: 41014562 DOI: 10.1016/j.xpro.2025.104115
Abstract

Research on inducing beige adipocytes to combat obesity and its comorbidities is growing, with stromal vascular fraction (SVF) from white adipose tissue showing potential to differentiate into both white and beige adipocytes. Here, we present a protocol for isolating the SVF from the iWAT (inguinal white adipose tissue) of mice and differentiating them into mature beige adipocytes. This protocol can be used to analyze the translational regulation and metabolic processes of beige adipocytes in vitro. For complete details on the use and execution of this protocol, please refer to Xu et al.1.

Keywords

Cell Differentiation; Cell culture; Cell isolation.

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