2. Academic Validation
  3. Aspergillus-Induced Cardiac Injury Modeling and Preventive Strategy Screening via a Universal Precise Cardiomyocyte-Based Electrophysiological Biosensing Platform

Aspergillus-Induced Cardiac Injury Modeling and Preventive Strategy Screening via a Universal Precise Cardiomyocyte-Based Electrophysiological Biosensing Platform

  • ACS Sens. 2025 Sep 24. doi: 10.1021/acssensors.5c00997.
Yumei Ge 1,  Ling Zou 2,  Jiajin Xue 3,  Duote Cai 3,  Yingwei Wang 4,  Haote Han 2 5,  Ning Hu 2 3
Affiliations

Affiliations

  • 1 Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.
  • 2 Department of Chemistry, Zhejiang-Israel Joint Laboratory of Self-Assembling Functional Materials, ZJU-Hangzhou Global Scientific and Technological Innovation Center, School of Medicine, Zhejiang University, Hangzhou 310058, China.
  • 3 General Surgery Department, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children's Health, Hangzhou 310052, China.
  • 4 Department of Laboratory Medicine, Tiantai People's Hospital of Zhejiang Province (Tiantai Branch of Zhejiang Provincial People's Hospital), Hangzhou Medical College, Taizhou, Zhejiang 317200, China.
  • 5 Key Laboratory of Reproductive Genetics (Ministry of Education) and Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
PMID: 40990321 DOI: 10.1021/acssensors.5c00997
Abstract

Aspergillus-induced cardiac injury represents a severe manifestation of invasive aspergillosis characterized by high mortality and diagnostic challenges. Current in vitro models fail to provide real-time monitoring of cardiac electrophysiological changes, which hinders the elucidation of the mechanisms underlying electrophysiological disturbances caused by Fungal invasion and its associated metabolites. Hence, this study developed a universal precise electrophysiological biosensing platform to enable real-time, dynamic monitoring of cardiomyocyte electrophysiological fluctuations under exposure to gliotoxin (GT), a major virulence factor of Aspergillus fumigatus. Acute exposure to 250 nM GT significantly increased the firing rate of cardiomyocytes within 8 h, while prolonged exposure progressively reduced the signal amplitude. Skullcapflavone II (SF2) can effectively protect against GT-induced cardiomyocyte damage by maintaining the electrophysiological signal stability. This self-developed platform provides a universal and precise tool to investigate the pathophysiology of Aspergillus-induced cardiac injury and to assess protective strategies through real-time electrophysiological monitoring.

Keywords

aspergillus-induced cardiac injury; electrophysiology biosensing; gliotoxin; preventive strategy screening; skullcapflavone II.

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