2. Academic Validation
  3. OT17 a novel microsatellite stable colorectal cancer cell line and organoid model for investigating BRAF V600E mutant tumorigenesis and targeted therapeutics

OT17 a novel microsatellite stable colorectal cancer cell line and organoid model for investigating BRAF V600E mutant tumorigenesis and targeted therapeutics

  • Biochem Biophys Rep. 2025 Sep 3:44:102235. doi: 10.1016/j.bbrep.2025.102235.
Chen Zheng 1 2 3,  Xuan Tang 4,  Shuang Wang 1 2 3,  Yuxuan Xu 1 2 3,  Keyang Jia 1 2 3,  Ganglong Gao 2,  Guiying Wei 5,  Gengming Niu 5,  Yiwen Wu 6,  Xiaozhe Qian 7,  Ying Zhu 8,  Dongxi Xiang 1 2 3 9
Affiliations

Affiliations

  • 1 State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai, PR China.
  • 2 Department of Biliary-Pancreatic Surgery, Department of Gastrointestinal Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
  • 3 Shanghai Key Laboratory for Cancer Systems Regulation and Clinical Translation (CSRCT), Shanghai, PR China.
  • 4 Department of Oncology, The Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200233, PR China.
  • 5 Shanghai OneTar Biomedicine, Shanghai, PR China.
  • 6 Beijing National Day School, Shanghai, PR China.
  • 7 Department of Thoracic Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
  • 8 Department of Oncology, Fengxian District Central Hospital, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiao Tong University, Shanghai, PR China.
  • 9 Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, PR China.
PMID: 40978200 DOI: 10.1016/j.bbrep.2025.102235
Abstract

Human Cancer cell lines serve as essential in vitro models for investigating tumor biology, carcinogenesis, molecular genetics, metastasis, and tumor evolution. In this study, we establish and characterize the OT17 cell line, derived from a moderately to poorly differentiated colorectal adenocarcinoma surgical specimen. Genetic analysis of OT17 revealed key mutations, including BRAF V600E, APC, and ERBB2, along with a deletion in TP53. Immunohistochemical profiling confirmed the expression of MLH1, MSH2, MSH6, and PMS2, indicating a microsatellite-stable (MSS) phenotype consistent with the primary tumor. The OT17 cell line demonstrated robust tumorigenicity in vivo, achieving a 100 % success rate in forming subcutaneous tumors in NOD-scid Il2rg-/-(NSG)(NSG) mice. Additionally, a corresponding 3D Organoid model (CO17) was established, which retained genetic concordance with OT17, further validating its relevance for preclinical applications. Together, the OT17 cell line and CO17 Organoid system represent robust models for advancing colorectal Cancer research, with potential applications in therapeutic screening, immunotherapy development, and MSS tumor biology studies.

Keywords

Cell line; Colorectal cancer; Organoid; Tumorigenicity.

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