2. Academic Validation
  3. Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control

Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control

  • MedComm (2020). 2025 Sep 12;6(9):e70354. doi: 10.1002/mco2.70354.
Joana Vitallé 1,  Sara Bachiller 1 2,  Beatriz Dominguez-Molina 1,  Eirini Moysi 3,  Sara Ferrando-Martínez 3,  María Inés Camacho-Sojo 1,  Isabel Gallego 1,  Alberto Pérez-Gómez 1,  María Reyes Jiménez-Leon 1,  Carmen Gasca-Capote 1,  Francisco José Ostos 1 2,  Mohammed Rafii-El-Idrissi Benhnia 1 2,  Laura E Via 4,  Antonio Mochón 5,  Luis Fernando López-Cortes 1,  Constantinos Petrovas 3 6,  Richard A Koup 3,  Ezequiel Ruiz-Mateos 1
Affiliations

Affiliations

  • 1 Institute of Biomedicine of Seville, IBiS/Virgen Del Rocío University Hospital/CSIC/University of Seville Clinical Unit of Infectious Diseases, Microbiology and Parasitology Seville Spain.
  • 2 Department of Medical Biochemistry Molecular Biology, and Immunology School of Medicine University of Seville Seville Spain.
  • 3 Tissue Analysis Core Immunology Laboratory Vaccine Research Center NIAID NIH Bethesda Maryland USA.
  • 4 Tuberculosis Research Section Laboratory of Clinical Immunology and Microbiology, and Tuberculosis Imaging Program Division of Intramural Research NIAID NIH Bethesda Maryland USA.
  • 5 Otorhinolaryngology Service Virgen Del Rocío University Hospital Seville Spain.
  • 6 Department of Laboratory Medicine and Pathology University of Lausanne University Hospital of Lausanne (CHUV) Lausanne Switzerland.
PMID: 40949488 DOI: 10.1002/mco2.70354
Abstract

Dendritic cells (DC) are known to modulate Antiviral immune responses; however, the knowledge about the role of different DC subsets in Antiviral T cell priming in human tissues remains uncompleted. In the context of HIV Infection, we determined the phenotype and location of plasmacytoid and CD141+ myeloid DCs (pDCs and mDCs) in lymph nodes of people living with HIV (PLWH). We found an interaction between pDCs and CD141+ mDCs with CD8+ T cells, being associated with participants' viral levels in blood and tissue. Moreover, we demonstrated a higher and more polyfunctional superantigen- and HIV-specific CD8+ T cell response after the coculture with Toll-like Receptor (TLR)-primed pDCs and CD141+ mDCs. Last, we showed the potential of programmed cell death-1 (PD-1) blocking using pembrolizumab to further increase antigen-specific CD8+ T cell response along with TLR agonists. Therefore, these results showed a cooperation between pDCs, CD141+ mDCs and CD8+ T cells in lymph nodes of PLWH, which is associated with higher HIV control, highlighting the importance of DC subsets crosstalk to achieve a more potent anti-HIV response and support the use of DC-based immunotherapies for HIV control.

Keywords

CD141 myeloid DC; CD8 T cell; HIV; dendritic cell (DC); lymph node; plasmacytoid DC.

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