2. Academic Validation
  3. ROS-Scavenging Multifunctional Microneedle Patch Facilitating Wound Healing

ROS-Scavenging Multifunctional Microneedle Patch Facilitating Wound Healing

  • Adv Healthc Mater. 2025 Aug 20:e01886. doi: 10.1002/adhm.202501886.
Mahshid Kharaziha 1 2,  Sahar Salehi 2,  Mahshid Shokri 1,  Seyed Mohsen Ahmadi Tafti 3 4,  Thomas Scheibel 2 5 6 7 8
Affiliations

Affiliations

  • 1 Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
  • 2 Department of Biomaterials, Faculty of Engineering Science, University of Bayreuth, 95447, Bayreuth, Germany.
  • 3 Colorectal Research Center, Imam Khomeini hospital complex, Tehran University of Medical Sciences, Tehran, Iran.
  • 4 Division of Colorectal Surgery, Department of Surgery, Tehran University of Medical Sciences, Tehran, Iran.
  • 5 Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), University of Bayreuth, 95447, Bayreuth, Germany.
  • 6 Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), University of Bayreuth, 95447, Bayreuth, Germany.
  • 7 Bayreuther Materialzentrum (BayMAT), University of Bayreuth, 95447, Bayreuth, Germany.
  • 8 Bayerisches Polymerinstitut (BPI), University of Bayreuth, 95447, Bayreuth, Germany.
PMID: 40832795 DOI: 10.1002/adhm.202501886
Abstract

Chronic wounds often experience delayed healing due to a disrupted microenvironment marked by persistent inflammation and excessive fibrosiswhich leads to tissue dysfunction. Excessive mitochondrial Reactive Oxygen Species (ROS) at the injury siteworsen this by hindering healing and promoting scarring. This study presents a multifunctional microneedle array (MNA) that combines ROS scavenging with accelerated tissue formation. The array contains Flightless I (Flii) siRNA-loaded arginine-functionalized poly (β-amino ester)-alginate (APA) particles within a hyaluronic acid methacrylate (HaMA) matrix. These siRNA-APA particles enhance ROS scavenging through nitric oxide (NO) delivery and silence Flii expression in HaCaT cells. The synergistic release of NO, arginine, and siRNA reduces pro-inflammatory gene expression (TNF-α and IL6), transforming M1 macrophages into the M2 phenotype and aiding the transition from inflammation to proliferation. Studies in a rat chronic wound model demonstrate that applying the siRNA-APA-laden MNA transdermally mitigates scar formation and promotes wound healing by reducing inflammatory responses. This siRNA-APA-laden HaMA MNA, with ROS scavenging and immunomodulatory activity, offers promising insights into effective chronic wound managementand wound dressing applications.

Keywords

ROS scavenging; arginine functionalized particles; flightless I siRNA; immunomodulatory properties; microneedle patch; wound healing.

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