2. Academic Validation
  3. Ethyl gallate isolated from Gypsophila licentiana Hand. -Mazz reduces the risk of liver injury by modulating the RAS pathway

Ethyl gallate isolated from Gypsophila licentiana Hand. -Mazz reduces the risk of liver injury by modulating the RAS pathway

  • J Ethnopharmacol. 2025 Aug 16;353(Pt B):120442. doi: 10.1016/j.jep.2025.120442.
Qinqin Zhang 1,  Haoyang Dai 2,  Chenxi Bu 3,  Zhining Gao 4,  Chengbo Hou 5,  Suiqing Chen 6
Affiliations

Affiliations

  • 1 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China. Electronic address: zhangqq2020123@163.com.
  • 2 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China. Electronic address: 17772837162@163.com.
  • 3 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China. Electronic address: buchenxi621@163.com.
  • 4 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China. Electronic address: 18737689617@163.com.
  • 5 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China. Electronic address: 18236817875@163.com.
  • 6 Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou, 450046, China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Jinshui East Road, Zhengzhou, 450046, China; Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan Province, China. Electronic address: chsq@hactcm.edu.cn.
PMID: 40825386 DOI: 10.1016/j.jep.2025.120442
Abstract

Ethnopharmacological relevance: Shanyin Chaihu (Gypsophila licentiana Hand. -Mazz) is a traditional Chinese medicine and possesses a history of use for the treatment of various diseases, including hepatitis. Ethyl gallate (EG), the natural pharmacologically active ingredient of Shanyin Chaihu, exerts obvious pharmacological effects against inflammation. However, the underlying therapeutic mechanisms of EG against ALI remain unknown.

Aim of the study: To investigate the role and elucidate the mechanisms of EG in ALI mice underlying by performing an integrated analysis.

Methods: EG was isolated and identified from Gypsophila licentiana Hand. -Mazz. The effects and action mechanisms of EG on ALI in mice were assessed using H&E staining, DIA proteomics, western blotting, immunohistochemistry, molecular dynamics, CETSA, and immunofluorescence.

Results: The results showed that EG was able to significantly decrease the liver coefficient, reduce the levels of ALT and AST in serum, attenuate the expression levels of ROS, F4/80 and LY6G in liver tissue. EG treatment significantly regulated the alteration of Ras signaling pathway in mice. In addition, EG had a good binding score with Ras. Furthermore, we added Ras agonists (ML-099) to mice and found that the effect of EG on improving liver injury was weakened. The experiment further proved that EG could target Ras.

Conclusion: These results suggest that EG has the vigour to alleviate liver injury in mice through Ras signaling. The findings provide a strategy for developing the EG, the compound isolated from Shanyin Chaihu, as a potential therapeutic agent for ALI.

Keywords

Acute liver injury; Ethyl gallate; Herbal therapy; RAS.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-124306
    99.75%, Ras-related GTPases Activator
    Ras