2. Academic Validation
  3. Sipeimine reduces ethanol-induced gastric ulcer in mice by suppressing Jak-Stat activation and restoring gut microbiota balance

Sipeimine reduces ethanol-induced gastric ulcer in mice by suppressing Jak-Stat activation and restoring gut microbiota balance

  • Sci Rep. 2025 Aug 6;15(1):28683. doi: 10.1038/s41598-025-12050-2.
Xia Yang 1,  Yue Li 2,  Bing Bai 2,  Qinlei Fan 3,  Fei Liu 3,  Shimin Xie 2,  Yaru Li 1,  Xiao Li 2,  Jicheng Han 2,  Yiquan Li 2,  Guangze Zhu 4,  Yilong Zhu 5,  Ningyi Jin 6 7 8
Affiliations

Affiliations

  • 1 Department of Pathology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 136200, Jilin, China.
  • 2 Key Laboratory of Jilin Province for Traditional Chinese Medicine Prevention and Treatment of Infectious Diseases, College of Integrative Medicine, Changchun University of Chinese Medicine, Changchun, 130117, PR China.
  • 3 Animal Health and Epidemiology center, Qingdao, 266000, China.
  • 4 Key Laboratory of Jilin Province for Traditional Chinese Medicine Prevention and Treatment of Infectious Diseases, College of Integrative Medicine, Changchun University of Chinese Medicine, Changchun, 130117, PR China. zhuguangze820@126.com.
  • 5 Key Laboratory of Jilin Province for Traditional Chinese Medicine Prevention and Treatment of Infectious Diseases, College of Integrative Medicine, Changchun University of Chinese Medicine, Changchun, 130117, PR China. zhuyilong66@163.com.
  • 6 Department of Pathology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 136200, Jilin, China. ningyik@126.com.
  • 7 Key Laboratory of Jilin Province for Traditional Chinese Medicine Prevention and Treatment of Infectious Diseases, College of Integrative Medicine, Changchun University of Chinese Medicine, Changchun, 130117, PR China. ningyik@126.com.
  • 8 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. ningyik@126.com.
PMID: 40770008 DOI: 10.1038/s41598-025-12050-2
Abstract

Long-term excessive alcohol intake can directly injure the gastroduodenal mucosa, causing gastric erosions, gastric ulcers, and gastrorrhagia. Fritillaria ussuriensis Maxim is a famous traditional Chinese medicine and health food produced in China. Sipeimine is an alkaloidal component of Fritillaria ussuriensis Maxim. This research aimed to investigate the protective effects of sipeimine on ethanol-induced gastric ulcers in mice. The results displayed that sipeimine could alleviate gastric tissue damage and decrease the levels of SOD, MDA, IL-6, IFN-γ, TNF-α, and IL-1β. Sipeimine treatment also adjusted macrophage polarization and the balance of Th17/Treg cell by reducing the expression of JAK1/2, p-Jak1/2, STAT1/3, and p-Stat1/3. Moreover, sipeimine could increase the abundance of Lactobacillus_johnsonii and decrease the abundance of Bacteroides_vulgatus in the gut microbiota. Meanwhile, sipeimine treatment significantly decreased the abundance of Rodentibacter_heylii and Streptococcus_cuniculi in the gastric microbiota. In conclusion, sipeimine can improve gastric ulcers by suppressing the Jak-Stat pathway, reversing gut-gastro microbiota dysbiosis, inhibiting macrophage M1 polarization, maintaining the balance of Th17/Treg cell, and lessening sustained inflammatory injury.

Keywords

Gastric ulcer; Gut-gastro microbiota; Jak-stat pathway; Sipeimine; Th17/Treg cell balance.

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