2. Academic Validation
  3. The mechanism of Yizhi Qingxin formula to improve neuroinflammation in Alzheimer's disease by regulating the TLR4/NF‑κB/NLRP3 pathway

The mechanism of Yizhi Qingxin formula to improve neuroinflammation in Alzheimer's disease by regulating the TLR4/NF‑κB/NLRP3 pathway

  • J Asian Nat Prod Res. 2025 Aug 4:1-19. doi: 10.1080/10286020.2025.2539987.
Wen-Xuan Chen 1 2,  Qiao-Yi Huang 1,  Jia-Hui Sun 2,  Xin-Yu Yang 1,  Hui Pei 1,  Yu Cao 1,  Hao Li 2,  Li-Na Ma 1
Affiliations

Affiliations

  • 1 Institute of Geriatrics, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • 2 Geriatrics Department, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
PMID: 40757693 DOI: 10.1080/10286020.2025.2539987
Abstract

Yizhi Qingxin formula (YQF) is a traditional Chinese medicine for the treatment of Alzheimer's disease (AD). This study explored the mechanism of YQF to improve AD-related neuroinflammation in vivo and in vitro. The behavioral tests, immunohistochemical analysis and Western blot were performed. The present results showed that YQF ameliorated cognitive deficits, reduced hippocampal Aβ deposition, and decreased the number of GFAP and Iba1-positive cells. The results also indicated that YQF suppressed neuroinflammatory cytokines (TNF-α, IL-1β, IL-6) by inhibiting TLR4/NF-κB/NLRP3 pathway. In summary, YQF provides a multi-targeted strategy for AD-related neuroinflammation, which may address limitations of single-pathway therapies.

Keywords

Alzheimer’s disease; NF-κB; NLRP3; TLR4; Yizhi Qingxin formula; neuroinflammation.

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