2. Academic Validation
  3. Autophagy suppression via SRC induction represents a therapeutic vulnerability for BAP1-mutant cancers

Autophagy suppression via SRC induction represents a therapeutic vulnerability for BAP1-mutant cancers

  • Autophagy. 2025 Aug 3:1-20. doi: 10.1080/15548627.2025.2535265.
Silvia Vega-Rubin-de-Celis 1,  Amanda Kristani 2 3 4,  Matthias Kudla 1,  Svenja Mergener 2 4,  Andrés Corrochano-Ruiz 2 4,  Safa Larafa 1,  Jetsy Montero-Vergara 1,  Laura-Marie Ahle 3 4,  Rainer Will 5,  Mael Lever 3,  Viktor Grünwald 6 7,  Boris Hadaschik 6,  Verena Jendrossek 1,  Nikolaos E Bechrakis 3,  Samuel Peña-Llopis 2 3 4
Affiliations

Affiliations

  • 1 Institute for Cell Biology (Cancer Research), University Hospital Essen, Essen, Germany.
  • 2 Translational Genomics, German Cancer Consortium (DKTK) at University Hospital Essen, Essen, Germany.
  • 3 Department of Ophthalmology, University Hospital Essen, Essen, Germany.
  • 4 German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • 5 Core Facility Cellular Tools, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • 6 Department of Urology, University of Duisburg-Essen, and German Cancer Consortium (DKTK), University Hospital Essen, Essen, Germany.
  • 7 Department of Medical Oncology, University Hospital Essen, Essen, Germany.
PMID: 40754831 DOI: 10.1080/15548627.2025.2535265
Abstract

BAP1 is a tumor suppressor and epigenetic modifier that is frequently mutated in Cancer, leading to increased aggressiveness and metastasis, as well as poor patient survival. Unfortunately, there are currently no specific therapies for metastatic tumors harboring BAP1 mutations. In this study, we have identified a new targetable BAP1-associated autophagic vulnerability. We demonstrate that BAP1 transcriptionally regulates the proto-oncogene Src, a non-receptor tyrosine kinase. Src then binds to, phosphorylates, and inactivates BECN1 (Beclin 1), an essential Autophagy protein. This inhibits Autophagy in cells derived from various Cancer types with BAP1 mutations. Treatment of these cells with Src inhibitors (such as dasatinib, bosutinib and saracatinib) and autophagy-inducing drugs (such as Tat-BECN1, SW076956 and SW063058) demonstrated a synergistic interaction between these compounds both in vitro and in ovo using a chick Chorioallantoic Membrane (CAM) assay. Furthermore, ex vivo studies employing patient-derived tumor organoids (PDTOs) of uveal melanoma (UM) and clear-cell renal cell carcinoma (ccRCC) as preclinical models have substantiated the synergism of these drugs, preferentially in the context of BAP1 loss. Our findings elucidate a novel BAP1-SRC-BECN1-autophagy regulatory axis that can be exploited therapeutically in precision oncology through the combination of Src inhibitors and Autophagy inducers, contingent upon patient stratification for BAP1 loss.Significance: Deadly cancers with BAP1 mutations suppress Autophagy by phosphorylating the Autophagy regulator BECN1 via the proto-oncogene Src. Treatment with Src inhibitors and Autophagy inducers exhibited synergism in vitro, in ovo and in patient-derived tumor organoids with BAP1 loss, paving the way for treating BAP1-deficient cancers with Autophagy inducers and kinase inhibitors.

Keywords

BRCA1-associated protein 1; PDOs; cholangiocarcinoma; patient-derived organoids; personalized medicine; tumoroids.

Figures
Products