2. Academic Validation
  3. Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning

Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning

  • Alzheimers Dement. 2025 Aug;21(8):e70331. doi: 10.1002/alz.70331.
Xiaoduo Zhao 1 2,  Jiayi Yu 1 3,  Bin Xu 1,  Zhi Xu 1,  Xia Lei 1,  Shilong Han 1,  Shangfei Luo 4 5 6,  Can Zhang 1,  Guoping Peng 7,  Jing Li 4 5 6,  Jie Yu 7,  Yi Ling 7,  Zhongqin Fan 1,  Wei Mo 8,  Ying Yang 1,  Jing Zhang 1 2 3 9
Affiliations

Affiliations

  • 1 Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 2 Nanhu Brain-computer Interface Institute, Hangzhou, Zhejiang, China.
  • 3 Lingang Laboratory, Shanghai, China.
  • 4 Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
  • 5 The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
  • 6 Innovation Research Center, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
  • 7 Department of Neurology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 8 Department of Psychiatry and Department of Immunology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Liangzhu Laboratory, Hangzhou, Zhejiang, China.
  • 9 National Human Brain Bank for Health and Disease, Zhejiang University, Hangzhou, Zhejiang, China.
PMID: 40731189 DOI: 10.1002/alz.70331
Abstract

Introduction: Growing evidence links gut microbiota (GM) to Alzheimer's disease (AD). Elevated lipopolysaccharide (LPS) levels, a Gram-negative bacteria component, are found in AD brains, but how LPS breaches the blood-brain barrier (BBB) remains unclear. Hypotheses suggest that bacteria-derived extracellular vesicles (bEVs) may transport LPS across the BBB.

Methods: bEVs were extracted from human and mouse feces and blood, and LPS levels were measured. In vivo imaging and immunofluorescence confirmed the transport of blood LPS-carrying bEVs across the BBB. The role of these bEVs in microglia was investigated both in vivo and in vitro.

Results: Elevated LPS-containing bEVs were detected in the plasma of AD patients compared to healthy individuals. These bEVs activated microglial Piezo1, consequently precipitating an excessive synaptic pruning process mediated by the C1q-C3 complement pathway.

Discussion: These findings illuminate the complex interplay between the gut microbiota, bEVs, neuroinflammation, and synaptic plasticity - a key early event in AD - offering insights for potential therapeutic interventions.

Highlights: GM-derived bEVs can traverse the BBB. LPS was necessary for bEVs' penetration into the brain, and bEVs might be closely related to AD progression. bEVs mediated microglial activation and synaptic pruning via C1q-C3 complement pathway. Microglia Piezo1 was involved in bEV-induced excessive synaptic pruning.

Keywords

Alzheimer's disease; C1q; Piezo1; bacteria‐derived extracellular vesicles; lipopolysaccharide; synaptic splicing.

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