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  3. Establishment of sika deer induced pluripotent stem cells

Establishment of sika deer induced pluripotent stem cells

  • Biol Reprod. 2025 Jul 28:ioaf166. doi: 10.1093/biolre/ioaf166.
Shuo Cao 1 2,  Fei Pan 1 2,  Ting Liu 1 2,  Lixia Zhao 1 2 3,  Qiqi Jiang 1 2,  Yanglin Chen 1 2,  Yongli Song 1 2,  Baojiang Wu 1 2,  Xihe Li 1 2 4,  Siqin Bao 1 2
Affiliations

Affiliations

  • 1 The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010020.
  • 2 Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot 010020.
  • 3 College of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.
  • 4 Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot 011517.
PMID: 40719351 DOI: 10.1093/biolre/ioaf166
Abstract

The sika deer (Cervus nippon) is a large ruminant species of significant commercial value, with antlers used in traditional medicine and meat prized for its nutritional and culinary qualities. In this study, we cultured fibroblasts from sika deer fetal fibroblasts (SDFs) and then reprogrammed them into sika deer induced pluripotent stem cells (SD-iPSCs) using nine exogenous factors (OCT4, SOX2, c-Myc, KLF4, NANOG, LIN28, RARG, LRH1 and LARGE T). The resulting SD-iPSCs exhibited to expressed key pluripotent genes, maintained genomic stability, and differentiated into the three germ layers in teratoma assays. Notably, we found that supplementing the mTeSR1 basal medium with XAV939, a Wnt/β-catenin pathway inhibitor, supported robust self-renewal and pluripotency maintenance of SD-iPSCs. Furthermore, using a bovine trophoblast stem cells (TSC) induction protocol, we successfully derived CDX2-positive trophoblast-like cells from SD-iPSCs. The establishment of SD-iPSCs not only offers a valuable model for studying mammalian pluripotent stem cells but also provides a versatile platform for biotechnological and translational research.

Keywords

Induced pluripotent stem cells (ipscs); Reprogramming; Sika deer; Trophoblast stem cells; Xav939.

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