2. Academic Validation
  3. Co-loaded simvastatin-ginsenoside Rh2 liposomes enhance cellular immune responses as vaccine adjuvants

Co-loaded simvastatin-ginsenoside Rh2 liposomes enhance cellular immune responses as vaccine adjuvants

  • Biochem Biophys Rep. 2025 Jul 15:43:102159. doi: 10.1016/j.bbrep.2025.102159.
Yinxiu Huang 1,  Qingqing Nie 2,  Zhijie Chen 2,  Wang Gong 2,  Wenqiang Cao 2,  Xue Wang 2,  Yanna Liu 2,  Ziyan Meng 2
Affiliations

Affiliations

  • 1 School of Modern Agricultural and Forestry Engineering, Ganzhou Polytechnic College, Ganzhou, Jiangxi Province, China.
  • 2 Key Laboratory of Green and Healthy Livestock and Poultry Breeding Technology of Jiangxi Provincial Department of Education, School of Animal Science, Ganzhou Polytechnic College, Ganzhou, Jiangxi Province, China.
PMID: 40697522 DOI: 10.1016/j.bbrep.2025.102159
Abstract

Vaccine adjuvants are pivotal in novel vaccine development, enhancing immunization and modulating immune responses. This study prepared liposomes loaded with simvastatin and ginsenoside Rh2 (LIPO-SIM-Rh2) via high-speed shearing, rotary evaporation, and high-pressure homogenization. Using C57BL/6J mice, six groups were established to explore its immune-enhancing effects and mechanisms. Following two-dose immunization at 0 and 21 days, IgG antibody titers (ELISA), immune cell responses (flow cytometry), and safety/function of antigen-presenting cells (pathological sections, immunofluorescence staining) were detected. Results showed LIPO-SIM-Rh2 had a stable dispersion and a near-100 % encapsulation efficiency. Compared with Other groups, it significantly increased anti-OVA specific IgG titer, promoted cellular immune response, up-regulated MHC-I molecule antigen presentation pathway, activated macrophages, promoted dendritic cell homing, and caused no obvious damage or inflammation to major organs. In conclusion, LIPO-SIM-Rh2 Adjuvant has a feasible preparation method and strong immune-enhancing potential for vaccines.

Keywords

Adjuvant; Cellular immunities; Ginsenoside Rh2; Liposome; Simvastatin; Vaccine.

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