2. Academic Validation
  3. Targeting BACH1/PSPH axis suppresses bladder cancer progression and gemcitabine resistance by downregulating S100A2 expression

Targeting BACH1/PSPH axis suppresses bladder cancer progression and gemcitabine resistance by downregulating S100A2 expression

  • Biochem Pharmacol. 2025 Jul 18:241:117182. doi: 10.1016/j.bcp.2025.117182.
Tingxuan Ma 1,  Jing Ma 2,  Yipin Wang 2,  Xintong Kui 1,  Jiaxin Liu 2,  Chuanchun Han 2,  Wei Wei 3,  Zhikun Lin 4,  Xishuang Song 5
Affiliations

Affiliations

  • 1 The First Affiliated Hospital, Dalian Medical University, Dalian 116044, PR China.
  • 2 Institute of Cancer Stem Cell, DalianMedical University, Dalian 116044, PR China.
  • 3 College of Medical Laboratory, Dalian Medical University, Dalian 116044, PR China. Electronic address: weiw01@dmu.edu.cn.
  • 4 The First Affiliated Hospital, Dalian Medical University, Dalian 116044, PR China. Electronic address: lzk19880125@163.com.
  • 5 The First Affiliated Hospital, Dalian Medical University, Dalian 116044, PR China. Electronic address: song-xishuang@163.com.
PMID: 40684997 DOI: 10.1016/j.bcp.2025.117182
Abstract

Increasing evidence indicates that phosphoserine Phosphatase (PSPH) promotes tumorigenesis in certain types of Cancer. However, its specific role and regulatory mechanism in bladder Cancer (BCa) remain unknown. In this study, we found that the expression level of PSPH is significantly upregulated in BCa tissues and patients with high PSPH levels exhibited shorter survival rates. Depletion of PSPH inhibited cell growth and metastatic potential of BCa cells. In contrast, upregulated PSPH promoted BCa progression and resistance to GEM. Mechanistically, increased PSPH enhanced the promoter activity of S100A2 and promoted S100A2 expression which led to malignant progression of BCa. The transcription factor BACH1 bound to the promoter of PSPH and facilitated PSPH expression in BCa. In addition, we also found that Brusatol directly bound to BACH1 and promoted BACH1 degradation. This study demonstrates the oncogenic role of PSPH in BCa and reveals that PSPH, upregulated by BACH1, promoted BCa progression and GEM resistance by elevating S100A2 expression. Brusatol acted as a novel inhibitor of BACH1 and suppressed malignance and GEM resistance of BCa by downregulating BACH1/PSPH/S100A2 pathway.

Keywords

BACH1; Bladder cancer; Brusatol; PSPH; S100A2.

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