2. Academic Validation
  3. PRMT5:MEP50 Are Mediators of Treatment-Induced Neuroendocrine Differentiation in Prostate Cancer

PRMT5:MEP50 Are Mediators of Treatment-Induced Neuroendocrine Differentiation in Prostate Cancer

  • Prostate. 2025 Sep;85(13):1196-1207. doi: 10.1002/pros.70006.
Hye Seung Nam 1,  Andrew Michael Asberry 1,  Xuehong Deng 1,  Sheng Liu 2 3,  Jiaoti Huang 4,  Chang-Deng Hu 1 5,  Jun Wan 2 3 6,  Michael K Wendt 1 5
Affiliations

Affiliations

  • 1 Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana, USA.
  • 2 Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • 3 Indiana University Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • 4 Department of Pathology, Duke University School of Medicine, Duke University, Durham, North Carolina, USA.
  • 5 Purdue Institute for Cancer Research, Purdue University, West Lafayette, Indiana, USA.
  • 6 Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
PMID: 40677010 DOI: 10.1002/pros.70006
Abstract

Background: Neuroendocrine prostate Cancer (NEPC) is a lethal subtype of prostate Cancer responsible for an estimated 20%-30% of castration-resistant prostate Cancer (CRPC) deaths. While NEPC can arise spontaneously, the majority of these cases emerge as treatment-induced NEPC (tNEPC). Our clinical and computational analyses identified increased expression of protein methyltransferase 5 (PRMT5) and its cofactor methylosome protein 50 (MEP50) in tNEPC.

Methods: Here we generated an in vitro Cell Culture and mouse model of prostate Cancer to recapitulate tNEPC induced upon treatment with the Androgen Receptor (AR) inhibitor, enzalutamide. The role of PRMT5 and its cofactor MEP50 were determined by overexpression. Depletion of these genes and pharmacological inhibition of PRMT5 were followed by analysis of cell viability, neurite growth, and effects on neuroendocrine-related gene transcription using immunofluorescence, immunohistochemistry, and Western blot. PRMT5 and MEP50 protein expression levels were comprehensively analyzed for clinical correlation in NEPC patient prostate tissue samples.

Results: Elevated PRMT5 and MEP50 correlated with increased recurrence in prostate Cancer patients receiving androgen deprivation therapy. Depletion of PRMT5 and MEP50 prevented neuroendocrine differentiation (NED)-induced by enzalutamide both in vitro and in a xenograft mouse model. Conversely, overexpression of PRMT5 and MEP50 was sufficient to induce NED in prostate Cancer cells. Evaluation of a genetically engineered mouse model, in which PRMT5 and MEP50 were overexpressed in the prostate, similarly indicated NEPC development.

Conclusions: Our data suggest that PRMT5:MEP50 are regulators of tNEPC. PRMT5/MEP50 expression could serve as a predictive biomarker and therapeutic target for aggressive forms of prostate Cancer.

Keywords

PRMT5; drug resistance; enzalutamide; neuroendocrine.

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