2. Academic Validation
  3. Lyophilized apoptotic vesicles restore DNA damage and mitochondria dysfunction to ameliorate radiation enteritis

Lyophilized apoptotic vesicles restore DNA damage and mitochondria dysfunction to ameliorate radiation enteritis

  • J Nanobiotechnology. 2025 Jul 16;23(1):521. doi: 10.1186/s12951-025-03592-8.
Bo Yang # 1,  Huigen Luo # 1,  Xutong Yan # 1,  Lingzhi Li 1,  Zhiqing Huang 1 2,  Yexiang Jiang 1,  Xueli Mao 1,  Songtao Shi 3 4 5,  Lan Ma 6 7
Affiliations

Affiliations

  • 1 Hospital of Stomatology, Guanghua School of Stomatology, South China Center of Craniofacial Stem Cell Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, 510055, China.
  • 2 Department of Temporomandibular Joint, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction & Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou, Guangdong, 510180, China.
  • 3 Hospital of Stomatology, Guanghua School of Stomatology, South China Center of Craniofacial Stem Cell Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, 510055, China. shisongtao@mail.sysu.edu.cn.
  • 4 International Center for Aging and Cancer (ICAC), Hainan Medical University, Haikou, Hainan, China. shisongtao@mail.sysu.edu.cn.
  • 5 Key Laboratory of Stem Cells and Tissue Engineering (Sun Yat-Sen University), Ministry of Education, Guangzhou, China. shisongtao@mail.sysu.edu.cn.
  • 6 Hospital of Stomatology, Guanghua School of Stomatology, South China Center of Craniofacial Stem Cell Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, 510055, China. malan6@mail.sysu.edu.cn.
  • 7 Department of Pediatric Dentistry, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, China. malan6@mail.sysu.edu.cn.
  • # Contributed equally.
PMID: 40671132 DOI: 10.1186/s12951-025-03592-8
Abstract

Radiation therapy for malignant tumor patients often induces radiation enteritis (RE), a condition that impairs their quality of life. Currently, there is no standard treatment regimen available. In this study, we used lyophilized apoptotic vesicles (Lpl-apoVs) from umbilical cord mesenchymal stem cells to treat RE in a murine model. We show that enema administration of lyophilized apoVs can ameliorate intestinal damage in RE mice. Mechanistically, Lpl-apoVs were internalized by intestinal endothelial cells (IECs) to alleviate radiation-induced DNA damage. In addition, Mitophagy was identified as a prerequisite for therapeutic efficacy, suggesting that rescue of DNA damage and restoration of mitochondrial quality are collaboratively to ameliorate RE diseased phenotypes. These findings indicate that enema administration of Lpl-apoVs is a novel strategy for RE therapy.

Graphical Abstract:

Supplementary Information: The online version contains supplementary material available at 10.1186/s12951-025-03592-8.

Keywords

Apoptotic vesicles; DNA damage repair; Lyophilization; Mitophagy; Radiation enteritis.

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