2. Academic Validation
  3. RBPMS2 can inhibit the NLRP3 / caspase-1 / GSDMD signaling pathway to resist pyroptosis in gastric cancer cells

RBPMS2 can inhibit the NLRP3 / caspase-1 / GSDMD signaling pathway to resist pyroptosis in gastric cancer cells

  • Sci Rep. 2025 Jul 1;15(1):21294. doi: 10.1038/s41598-025-01125-9.
Nailin Zhang 1 2,  Lina Yan 3,  Hua Cao 4,  Nana Zhang 5,  Tong Zhang # 6,  Shengjiang Guan # 7,  Qiquan Liu # 8 9
Affiliations

Affiliations

  • 1 Department of Spleen and Stomach Diseases, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, 050000, China.
  • 2 Department of Spleen and Stomach Diseases, Hebei Key Laboratory of Turbidity Toxin Syndrome, Hebei University of Chinese Medicine, Shijiazhuang, 050000, China.
  • 3 School of Public Health, Hebei Medicine University, Shijiazhuang, 050000, China.
  • 4 Graduate school, Hebei University of Chinese Medicine, Shijiazhuang, 050000, China.
  • 5 Respiratory department, The Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Changan District, Shijiazhuang, 050011, China.
  • 6 Respiratory department, The Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Changan District, Shijiazhuang, 050011, China. doctong1990@163.com.
  • 7 Pharmaceutical Department, Hebei Provincial Hospital of Chinese Medicine, No.389 Zhongshan Road, Changan District, 050011, Shijiazhuang, Hebei, China. guanshengj1@hebcm.edu.cn.
  • 8 Department of Spleen and Stomach Diseases, Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang, 050000, China. Liuqq56@163.com.
  • 9 Pharmaceutical Department, Hebei Provincial Hospital of Chinese Medicine, No.389 Zhongshan Road, Changan District, 050011, Shijiazhuang, Hebei, China. Liuqq56@163.com.
  • # Contributed equally.
PMID: 40595708 DOI: 10.1038/s41598-025-01125-9
Abstract

Gastric Cancer (GC) is one of the most common malignancies worldwide, and its development is closely associated with the abnormal expression of numerous genes. RNA - binding protein with multiple splicing 2 (RBPMS2), a member of the RNA - binding protein family, has recently been found to be abnormally activated in GC cells. Meanwhile, Pyroptosis, a form of programmed cell death, is related to tumorigenesis, development, and immune responses.This study investigated the role of the RNA - binding protein RBPMS2 in GC. It revealed the high expression of RBPMS2 in GC cells and its association with poor prognosis. RBPMS2 promoted the proliferation, invasion, and migration of GC cells while inhibiting Pyroptosis by suppressing the NLR family pyrin domain containing 3 (NLRP3)/Caspase - 1/gasdermin (DGSDMD) signaling pathway. Knocking out RBPMS2 activated Pyroptosis, leading to cell membrane damage and increased expression of Pyroptosis - related proteins. The NLRP3 Inhibitor MCC950 reversed these effects, confirming the involvement of this pathway. These findings suggest that RBPMS2 may be a potential therapeutic target for inducing Pyroptosis in gastric Cancer cells, providing novel insights into treatment strategies for gastric Cancer.

Keywords

Cell migration; Cell multiplication; Ferroptosis; Gastric cancer; Pyrodeath; RBPMS2.

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